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首页> 外文期刊>Journal of Virology >Morphogenesis of foot-and-mouth disease virus. I. Role of procapsids as virion Precursors.
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Morphogenesis of foot-and-mouth disease virus. I. Role of procapsids as virion Precursors.

机译：口蹄疫病毒的形态发生。 I. Pronapsids作为病毒群岛前体的作用。

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The role of procapsids during foot-and-mouth disease virus multiplication was studied on infected BHK-21 cells. Purified virus and procapsids were obtained by treating the infected cytoplasmic extracts with RNase and EDTA. The synthesis of virus, procapsids, and total particles was determined in pulse-chase experiments. A precursor-product relationship between procapsids and virions was obtained. The results show that the rate of synthesis of total particles (virus + procapsids) was linear from the addition of the label and was identical to that corresponding to virions. Therefore, the speed of the morphogenetic process as well as the existence of a precursor pool of structural proteins was established. Furthermore, the rate of virus synthesis from procapsids was identical to the rate of synthesis of procapsids from their structural precursors. A quantitative recovery of label from procapsids into virions was obtained by the use of cycloheximide or tosyl-lysine chloromethyl ketone. Under these conditions, virus synthesis proceeds, indicating that these drugs do not affect the morphogenetic step studied in this paper.
机译：在感染的BHK-21细胞上研究了催乳素病毒在口蹄疫病毒倍增过程中的作用。通过用RNase和EDTA治疗受感染的细胞质提取物来获得纯化的病毒和催乳素体。在脉冲追踪实验中测定病毒，假膜和总粒子的合成。获得proCapsids和病毒粒子之间的前体 - 产品关系。结果表明，总颗粒（病毒+假胶）的合成率从添加标签中的线性，与对应于病毒粒子相同。因此，建立了形态发生过程的速度以及结构蛋白的前体池的存在。此外，来自proPapsids的病毒合成率与来自其结构前体的催盲率合成的速率相同。通过使用环己酰亚胺或甲硅烷基丙氨酸氯甲基酮获得，从Puropapsids的定量恢复到病毒粒中。在这些条件下，病毒合成进行，表明这些药物不会影响本文中研究的形态发生步骤。

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