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首页> 外文期刊>Journal of Virology >Electron microscopic study of measles virus infection: unusual antibody-triggered redistribution of antigens on giant cells.
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Electron microscopic study of measles virus infection: unusual antibody-triggered redistribution of antigens on giant cells.

机译：电子显微镜研究麻疹病毒感染：巨细胞上抗原的异常抗体触发再分布。

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Vero cells infected with measles virus fuse to form multinucleated cells which incorporated virus-specific antigens in their membrane. The distribution of these antigens was analyzed after a brief treatment with human anti-measles immunoglobulin G, using autoradiography and immunoperoxidase labeling combined with transmission and scanning electron microscopy. Virs-specific antigens were distributed over the entire surface of giant cells treated at 4 degrees C with human anti-measles immunoglobulin G and labeled Protein A. When cells were shifted to 37 degrees C, labeled antigen-antibody complexes were redistributed in two stages. Patch formation occurred in 5 to 15 min. Later, antigen-antibody complexes became concentrated in a paracentral "ring" rather than typical caps. Patch formation occurred in the presence of metabolic inhibitors, whereas ring formation was inhibited by metabolic inhibitors. These rings contained membrane folds, villi, and viral buds, whereas the rest of the membrane was smooth. In addition, shedding, endocytosis of antigen-antibody complexes, and reexpression of antigens were observed. Antibodies to nonviral membrane antigens induced the same pattern of redistribution. Infected cells treated with anti-measles Fab' fragments maintained a homogenous distribution of label throughout the experiments. In conclusion, intact immunoglobulins, but not Fab' fragments, were able to induce a dramatic redistribution of viral antigen on the membrane of giant cells infected with measles virus.
机译：Vero细胞感染麻疹病毒保险丝以形成多核细胞，其在其膜中掺入病毒特异性抗原。在用抗动脉造影和免疫氧化曲酶标记与透射和扫描电子显微镜结合后，分析了这些抗原的分布。将特异性抗原分布在用4℃处理的巨细胞的整个表面上，用人抗麻疹免疫球蛋白G和标记的蛋白质A。当细胞移至37℃时，标记的抗原 - 抗体复合物在两个阶段重新分布。贴片形成发生在5至15分钟内。后来，抗原抗体复合物浓缩成高级“环”而不是典型的帽。在存在代谢抑制剂存在下发生蛋白形成，而通过代谢抑制剂抑制环状。这些环含有膜折叠，绒毛和病毒芽，而剩余的膜是光滑的。此外，观察到抗原，抗原抗体复合物的内吞作用和抗原的重新抑制。对非血管膜抗原的抗体诱导相同的再分配模式。用抗衡杆Fab'片段处理的受感染的细胞在整个实验中保持了标记的均匀分布。总之，完整的免疫球蛋白，但不是Fab'片段，能够在感染麻疹病毒感染的巨细胞膜上诱导病毒抗原的显着再分布。

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