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首页> 外文期刊>Journal of Virology >Characterization of Measles Virus-Specific Proteins Synthesized In Vivo and In Vitro from Acutely and Persistently Infected Cells
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Characterization of Measles Virus-Specific Proteins Synthesized In Vivo and In Vitro from Acutely and Persistently Infected Cells

机译：急性和持续受感染细胞体内和体外合成的麻疹病毒特异性蛋白质的表征

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Measles virus protein synthesis has been analyzed in acutely and persistently infected cells. To assess the role of measles in subacute sclerosing panencephalitis (SSPE), measles viral proteins synthesized in vivo or in vitro were tested for reactivity with serum from a guinea pig(s) immunized with measles virus and sera from patients with SSPE. Guinea pig antimeasles virus serum immunoprecipitates the viral polypeptides of 78,000 molecular weight (glycosylated [G]), 70,000 molecular weight (phosphorylated [P]), 60,000 molecular weight (nucleocapsid [N]), and 35,000 molecular weight (matrix [M]) from cells acutely infected with measles virus as well as from chronically infected cells, but in the latter case, immunoprecipitated M protein has a reduced electrophoretic migration. Sera of SSPE patients immunoprecipitated all but the G protein in acutely infected cells and only the P and N proteins from chronically infected cells. In immunoprecipitates of viral polypeptides synthesized in a reticulocyte cell-free translation system, in response to mRNA from acutely or persistently infected cells, the 78,000-molecular-weight form of the G protein was not detected among the cell-free products of either mRNA. Guinea pig antimeasles virus serum immunoprecipitated P, N, and M polypeptides from the products of either form of mRNA, whereas SSPE serum immunoprecipitated the P and N polypeptides but not the M polypeptide. The differences in immunoreactivity of the antimeasles virus antiserum and the SSPE serum are discussed in terms of possible modifications of measles virus proteins in SSPE.
机译：已经在急性和持续的感染细胞中分析了麻疹病毒蛋白质合成。为了评估麻疹在亚急性硬化的终连脑炎（SSPE）中的作用，在体内或体外中合成的麻疹病毒蛋白与来自用SSPE患者免疫的豚鼠免疫的豚鼠的血清进行反应性。豚鼠抗体病毒血清免疫沉淀78,000分子量的病毒多肽（糖基化[g]），70,000分子量（磷酸化[p]），60,000个分子量（核衣壳[n]），和35,000个分子量（基质[m]）从敏锐地感染麻疹病毒以及从慢性感染的细胞感染，但在后一种情况下，免疫沉淀的M蛋白具有降低的电泳迁移。 SSPE患者的Sera免疫沉淀，除了急性感染细胞中的G蛋白，并且仅来自慢性感染细胞的P和N蛋白。在分键细胞无细胞翻译系统中合成的病毒多肽的免疫沉淀物中，响应于来自急性或持续感染的细胞的mRNA，在任一mRNA的无细胞产物中未检测到78,000分子量形式的G蛋白。豚鼠抗体血清免疫沉淀的P，N和M多肽来自任一形式的mRNA，而SSPE血清免疫沉淀的P和N多肽但不是M多肽。就SSPE中的麻疹病毒蛋白的可能修饰而言，讨论了抗体病毒抗血清和SSPE血清的免疫反应性的差异。

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