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首页> 外文期刊>Journal of Virology >T-antigen expression in proliferating and non-proliferating simian virus 40-transformed mouse cells.
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T-antigen expression in proliferating and non-proliferating simian virus 40-transformed mouse cells.

机译：T-抗原在增殖和非增殖血清病毒40转化的小鼠细胞中的表达。

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Previous studies with simian virus 40-transformed mouse 3T3 cells which are temperature sensitive for the expression of the transformed phenotype (ts SV3T3 cells) have shown that T-antigen expression and viral DNA transcription are under cell cycle control. Using these ts SV3T3 cells, we studied the expression of the viral genome under proliferating and non-proliferating conditions, in the presence and absence of inhibitors of macromolecular synthesis and of the tumor promoter phorbol myristate acetate. ts SV3TE cells which are growth arrested at 39 degrees C by low serum concentration or saturation density accumulated in G1 and did not express T-antigen. When these cells were induced to proliferate, at either 32 or 39 degrees C, T-antigen synthesis preceded the entry of the cells into the S-phase and was not coupled to DNA replication. G1-arrested ts SV3T3 cells were induced to synthesize T-antigen by phorbol myristate acetate treatment, but T-antigen alone was not sufficient to induce cellular DNA synthesis. Isoleucine deprivation arrested growth of ts SV3T3 cells, but these cells, as well as normal 3T3, did not accumulate in G1 and continued to express T-antigen. The temperature-sensitive expression of the transformed phenotype in the ts SV3T3 cells does not appear to be due to a lack of transcription of specific regions of the integrated simian virus 40 genome at 39 degrees C.
机译：以前的研究与Simian病毒40转化的小鼠3T3细胞，其对转化的表型（TS SV3T3细胞）表达的温度敏感表明，T-抗原表达和病毒DNA转录是在细胞周期控制下。使用这些TS SV3T3细胞，我们研究了在大分子合成的抑制剂和肿瘤促进剂猪肉豆蔻酸盐的存在和不存在下，研究了病毒基因组的表达和不增殖条件。 TS SV3TE细胞通过在G1中累积的低血清浓度或饱和密度，在39℃下生长并未表达T-抗原。当诱导这些细胞增殖时，在32或39℃下，T-抗原合成在细胞进入S相中并未偶联至DNA复制。诱导G1捕获的TSSV3T3细胞通过Phorbol Myristate醋酸酯处理合成T-抗原，但仅T-抗原不足以诱导细胞DNA合成。异氨酸剥夺被阻止TS SV3T3细胞的生长，但这些细胞以及正常的3T3，在G1中没有积聚并继续表达T-抗原。 TS SV3T3细胞中转化的表型的温度敏感表达似乎不是由于在39摄氏度下缺乏综合硅藻病毒40基因组的特定区域的转录。

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