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首页> 外文期刊>Journal of Virology >Mutant of B-tropic murine leukemia virus synthesizing an altered polymerase molecule.
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Mutant of B-tropic murine leukemia virus synthesizing an altered polymerase molecule.

机译：B-Tropic鼠白血病病毒的突变体合成改变的聚合酶分子。

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A nonconditional mutant of B-tropic murine leukemia virus (MuLV), defective in polymerase, has been isolated by cloning chronically infected cells. The cell clone containing the mutant produced virus particles which were noninfectious. However, superinfection of the cells by replication-competent XC-negative viruses resulted in the rescue of virus capable of forming plaques in a modified XC test, termed the "complementation plaque assay" (A. Rein and R. H. Bassin, J. Virol. 28:656-660, 1978). Analysis of the noninfectious virions produced without superinfection demonstrated that they contained only 2 to 5% of the wild-type level of reverse transcriptase activity. Purification of this activity indicated that it was associated with a smaller molecule than that produced by wild-type virus. Cells producing the mutant virions did not contain the gag-pol precursor, Pr180gag-pol; however the cells contained proteins of 147K and 114K daltons precipitable with anti-pol serum. All of the normal structural proteins as well as 70S genomic RNA could be detected in the mutant particles. An interference test indicated that a functional ecotropic glycoprotein was synthesized by the mutant. These results indicate that the mutant has a unique defect in the pol gene.
机译：通过克隆慢性感染的细胞，分离了聚合酶中有缺陷的B-热带鼠白血病病毒（Mulv）的非条件突变体。含有突变体产生的病毒颗粒的细胞克隆是非缺陷的。然而，通过复制态态XC阴性病毒的细胞的SuperInfect导致能够在改性XC测试中形成斑块的病毒，称为“互补斑块测定”（A.ReN和Rh Bassin，J.Virol。28 ：656-660,1978）。没有过度染色的非缺陷病毒粒子的分析证明它们仅包含2％至5％的野生型逆转录酶活性。该活性的纯化表明它与较小的分子相关，而不是通过野生型病毒产生的分子。产生突变病毒藻的细胞不含Gag-Pol前体PR180GAG-POL;然而，细胞含有147K和114K道尔顿的蛋白质，与抗Pol血清可脱蛋白。所有正常结构蛋白以及70s基因组RNA可以在突变颗粒中检测。干扰试验表明，突变体合成了功能性生态糖蛋白。这些结果表明突变体在POL基因中具有独特的缺陷。

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