• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Effect of alkylation on the physical properties of simian virus 40 T-antigen species.
【24h】

Effect of alkylation on the physical properties of simian virus 40 T-antigen species.

机译：烷基化对猿猴病毒40T-抗原物种物理性质的影响。

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We analyzed large and small species of T-antigen by immunoprecipitation and two-dimensional gel electrophoresis. The T-antigen species were subjected to electrophoresis either directly or after reduction and alkylation with N-ethylmaleimide. Treatment with N-ethylmaleimide improved the resolution of large-T by two-dimensional gel electrophoresis and was a requirement for the resolution of small-t antigen on two dimensional gels. Large-T did not form a discrete protein spot, but rather formed a streak from approximately pH 6.5 to 6.9 on isoelectric focusing gels. Small-t formed a sharp protein spot at approximately pH 7.2 when subjected to electrophoresis under non-equilibrium conditions which extended the pH gradient to include proteins with basic isoelectric points. Treatment with N-ethylmaleimide decreased the mobility of the T-antigen species during sodium dodecyl sulfate gel electrophoresis. We suggest that the apparent increase in molecular weight was due to the association of N-ethylmaleimide with cysteine-rich regions of these proteins. Viable deletion mutants of simian virus 40 which do not induce the synthesis of small-t but product small-t-related polypeptides were used to localize the cysteine-rich region of small-t to between 0.54 and 0.59 on the genetic map of simian virus 40.
机译：通过免疫沉淀和二维凝胶电泳，通过分析大而小的T-抗原物种。将T-抗原物种直接或在用N-乙基马来酰亚胺的还原和烷基化后进行电泳。用N-乙基马来酰亚胺处理通过二维凝胶电泳改善大T的分辨率，是在二维凝胶上分辨小T抗原的要求。大T未形成离散的蛋白质点，而是在等电聚焦凝胶上从大约pH 6.5到6.9形成条纹。当在延伸pH梯度的非平衡条件下进行电泳时，小-t在大致pH 7.2下形成尖锐的蛋白质点，其包括具有基本等电点的蛋白质。用N-乙基马来酰亚胺处理降低了在十二烷基硫酸钠凝胶电泳期间T-抗原物种的迁移率。我们表明分子量的表观增加是由N-乙基马来酰亚胺与这些蛋白质的富含半胱氨酸的区域的结合。 SIMIAN病毒40的可行缺失突变体，其不诱导小T但产品的小T相关多肽用于将富含半胱氨酸的小T至0.54和0.59的血清病毒的遗传图谱局部化40。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号