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首页> 外文期刊>Journal of Virology >Two avian sarcoma virus mutants with defects in the DNA polymerase-RNase H complex.
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Two avian sarcoma virus mutants with defects in the DNA polymerase-RNase H complex.

机译：两种禽Sarcoma病毒突变体，具有DNA聚合酶-RNase H络合物的缺陷。

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Two mutants of avian sarcoma virus which exhibit different phenotypes have been analyzed for the properties of their RNA-dependent DNA polymerase and RNase H activities. LA 338 is a complex multiple mutant with at least one lesioneach in transformation and replication functions. The purified RNA-dependent DNA polymerase-RNase H complex from the mutant is twofold more thermolabile than that from the wild-type parent. A peculiarity of this mutant is that the ability of the enzyme to respond to synthetic template-primers is lost more rapidly than is the response to native RNA as template. The mutant enzyme cannot be protected from inactivation by the addition of synthetic template-primers. LA 672 represents a different phenotype among reverse transciptase mutant, showing a "late"-acting block in replication which affects only production of progeny by infected cells grown at the nonpermissive temperature. The purified DNA polymerase-RNase H complex of LA 672 is not thermolabile; rather, progeny grown at the nonpermissive temperature yield purified enzyme with a 20-fold-reduced specific activity in both DNA polymerase and RNase H. The content of reverse transcriptase protein in such noninfectious progeny, furthermore, did not appear to be significantly diminished since immunologically active enzyme could be demonstrated in a competition test for anti-reverse transcriptase antibody and since beta and alpha subunits of reverse transcriptase could be identified after polyacrylamide gel electrophoresis of partially purified enzyme preparations. The amounts of beta and alpha from the mutant were about twofold lower.
机译：已经分析了表现出不同表型的禽Sarcoma病毒的两个突变体，用于其RNA依赖性DNA聚合酶和RNase H活性的性质。 La 338是一种复杂的多个突变体，其具有至少一种转化和复制功能。从突变体的纯化的RNA依赖性DNA聚合酶-RNase H络合物比来自野生型母体的热电子更加热。该突变体的特殊性是酶对合成模板引物的能力更快地丧失，而不是对天然RNA作为模板的反应。不能通过加入合成模板引物来保护突变酶免受灭活。 La 672表示逆转录酶突变体中的不同表型，显示了在非智能温度下生长的受感染细胞仅影响后代的“晚期”的嵌段。 La 672的纯化的DNA聚合酶-RNase H络合物不是散热器;相反，在非智能温度产率纯化的酶中生长的后代在DNA聚合酶和RNase H中具有20倍降低的特异性活性。此外，这种无染性后代的逆转录酶蛋白的含量并未显着降低活性酶可以在抗逆转录酶抗体的竞争试验中证明，因为在部分纯化的酶制剂的聚丙烯酰胺凝胶电泳后，可以鉴定逆转录酶的β和α亚基。来自突变体的β和α的量约为双重。

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