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首页> 外文期刊>Journal of Virology >Deoxythymidine nucleotide metabolism in Bacillus subtilis W23 infected with bacteriophage SP1Oc: preliminary evidence that dTMP in SP10c DNA is synthesized by a novel, bacteriophage-specific mechanism.
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Deoxythymidine nucleotide metabolism in Bacillus subtilis W23 infected with bacteriophage SP1Oc: preliminary evidence that dTMP in SP10c DNA is synthesized by a novel, bacteriophage-specific mechanism.

机译：枯草硫氰胺核苷酸代谢在枯草芽孢杆菌W23感染噬菌体SP1oC：初步证据表明SP10C DNA中的DTMP通过新颖的噬菌体特异性机制合成。

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Despite the fact that mature SP10c DNA contains dTMP, the acid-soluble fraction of infected cells contained no dTTP during the interval of phage replication. However, infected cells contained normal cellular levels of dATP, dGTP, and dCTP. Upon infection of deoxythymidine-starved Bacillus subtilis M160 (a deoxythymidine-requiring mutant of B. subtilis W23), mature phage DNA with a normal dTMP content was made. SP10c codes for an enzyme that seems to catalyze the tetrahydrofolate-dependent transfer of 1-carbon fragments to the 5 position of dUMP. The transfer of 1-carbon fragments is not accompanied by oxidation of tetrahydrofolage to dihydrofolate, implying that the enzyme in question is not a dTMP synthetase. It is proposed that dTMP in mature SP10c DNA is derived by the postreplicational modification of some other nucleotide and not by the direct incorporation of dTTP into DNA.
机译：尽管成熟的SP10C DNA含有DTMP，但在噬菌体复制的间隔期间感染细胞的酸可溶性级分含有DTTP。然而，受感染的细胞含有DATP，DGTP和DCTP的正常细胞水平。在感染脱氧硫氨酸饥饿的枯草芽孢杆菌M160（需要B.枯草芽孢杆菌W23的脱氧硫化汀突变体），制备了具有正常DTMP含量的成熟噬菌体DNA。用于酶的SP10C码似乎催化1-碳碎片的四氢醇依赖性转移到倾卸5个位置。 1-碳碎片的转移不伴随四氢硅氧化物至二氢氢醇，这意味着所讨论的酶不是DTMP合成酶。提出了成熟SP10C DNA中的DTMP通过对一些其他核苷酸的搏动修饰来源的，而不是通过直接掺入DTTP进入DNA。

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