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首页> 外文期刊>Nucleic acids research >Identification and mapping of N6-methyladenosine containing sequences in Simian Virus 40 RNA
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Identification and mapping of N6-methyladenosine containing sequences in Simian Virus 40 RNA

机译：含有Simian病毒40RNA序列的N6-甲基碳糖苷序列的鉴定和映射

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Late SV4O 16S and 19S mRNAs were found to contain an average of three m6A residues per mRNA molecule. The methylated residues of both the viral and cellular mRNAs occur in two sequences; Gpm6ApC and (Ap)nm6ApC where n = 1–4. More than 60% of the m6A residues in SV4O 16S and 19S mRNAs occur in Gpm6ApC even though there are twice as many (A)nAC than GAC sequences in these messengers. The m6A containing oligonucleotides of late SV40 mRNAs were localized in the viral messengers. In the 16S mRNA two m6A oligonucleotides were located at the 5′ coding region between 0.95–0.0 map units. The third m6A residue was mapped between 0.0–0.14 nap units in the translated portion of this mRNA. The overall pattern of internal methylation in the 19S mRNA is similar. However, some differences between 16S and 19S mRNAs were observed in both the content and location of the longer (Ap)nm6ApC nucleotides These results provide the first example of precise localization of internal methylation sequences in mRNA species with defined coding specificity. It implies that a) location of m6A residues is not random but specific to a particular region of the RNA, b) apart from sequence specificity other structural features of the mRNA may influence internal methylation and c) m6A residues are present in coding regions of SV40 mRNAs.
机译：发现SV4O 16S和19S MRNAS的平均每MRNA分子的平均值为3μm 6 / sup>残留物。病毒和细胞mRNA的甲基化残基发生在两个序列中; GPM 6 apc和（ap）nm 6 apc，其中n = 1-4。 SV4O 16S和19S MRNA中的超过60％的M 6 残留物在GPM 6 APC中发生，即使存在两倍（a） n < / sub> ac比这些信使的GAC序列。 SV40后期MRNA晚期MRNA的含寡核苷酸的m 6 局部化在病毒使者中。在16s mRNA中，寡核苷酸在0.95-0.0映射单元之间的5'编码区位于5'编码区域。将第三M 6 残基在该mRNA的平移部分中的0.0-0.14个午点单元之间映射。 19S mRNA中的内部甲基化的整体模式类似。然而，在较长（ap） n m 6 apc核苷酸的含量和位置，观察到16s和19s mRNA之间的一些差异这些结果提供了精确的第一个例子mRNA物种中内部甲基化序列的定位，具有定义的编码特异性。它意味着a）m 6 残基的位置不是随机但特异于RNA，b）的特定区域，除序列特异性外，mRNA的其他结构特征可能影响内部甲基化和c） M 6 残留物存在于SV40 mRNA的编码区域中。

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