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Semiconservative synthesis of single-stranded RNA by bacteriophage phi 6 RNA polymerase.

机译:通过噬菌体PHI 6 RNA聚合酶半链RNA的半导体合成。

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The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bacteriophage phi 6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture synthesized at least one round of large, medium, and small single-stranded RNA molecules, respectively. Some of these particles reinitiated synthesis such that an average of 1.5 large, 33 medium, and 24 small single-stranded RNAs were synthesized from each double-stranded RNA.
机译:通过半透明(位移)机制,Pseudomonas poplaceolicola噬菌体Phi 6的核衣壳中的RNA聚合酶从病毒双链RNA基因组转录大,培养基和小单链RNA。测定混合物中约23%,63%和65%的核衣壳颗粒分别合成至少一轮大,培养基和小单链RNA分子。这些颗粒中的一些重新加强了合成,使得平均为1.5大,33培养基和24个小单链RNA,从每个双链RNA合成。

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