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首页> 外文期刊>Journal of Virology >Relationship of retroviruses isolated from human leukemia tissues to the woolly monkey-gibbon ape leukemia viruses.
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Relationship of retroviruses isolated from human leukemia tissues to the woolly monkey-gibbon ape leukemia viruses.

机译：从人白血病组织中分离的逆转录病毒与羊毛猴 - 长臂猿白血病病毒的关系。

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Retroviruses have been isolated from the tissues of human leukemia patients. Previous studies have shown that these isolates share some antigenic determinants with the family of viruses isolated from the woolly monkey and gibbon ape and that they exhibit partial nuclei acid homology with this same group of viruses. We have compared the RNAs of the viruses by two-dimensional polyacrylamide gel electrophoresis of the large RNase T1-resistant oligonucleotides. The degree of sequence identity between the RNAs was determined by the similarity of their RNase T1-resistant oligonucleotide pattern on gels, fingerprints, and in some cases by partial sequence analysis of individual oligonucleotides. This technique permits us to determine the degree of sequence identity among related RNA species. From our studies we conclude that viruses isolated from the tissues of two human leukemia patients, A1476 and SKA 21-3, as well as some subcultures of a virus isolated from the leukemic tissues of a third patient, HL23V, are closely related to the wooly monkey virus. However, the fingerprints of other HL23 viral isolates are very similar to that of GaLVSF, a gibbon ape leukemia virus isolated from a lymphosarcoma.
机译：逆转录病毒已从人白血病患者的组织中分离出来。以前的研究表明，这些分离株与羊毛猴和长臂猿分离的病毒家族共享一些抗原决定因素，并且它们与同一组病毒表现出部分核酸同源物。通过大RNaseT1抗性寡核苷酸的二维聚丙烯酰胺凝胶电泳与病毒的RNA与病毒的RNA进行了比较。 RNA之间的序列同一程度由其RNaseT1抗性寡核苷酸图案的相似性在凝胶，指纹，以及在一些情况下通过各个寡核苷酸的部分序列分析来确定。该技术允许我们确定相关RNA种类之间的序列同一性程度。从我们的研究来，我们得出结论，从两人白血病患者的组织中分离的病毒，A1476和SKA 21-3，以及来自第三患者的白血病组织HL23V的病毒的一些潜水物与羊毛密切相关猴子病毒。然而，其他HL23病毒分离物的指纹非常类似于从淋巴瘤中分离的长臂猿白血病病毒的Galvsf。

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