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首页> 外文期刊>Journal of Virology >Heterogeneity of the 3' end of minus-strand RNA in the poliovirus replicative form.
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Heterogeneity of the 3' end of minus-strand RNA in the poliovirus replicative form.

机译：在Poliovirus复制形式中的3'末端的束末端末端的异质性。

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The 3' terminus of the strand (minus strand) complementary to poliovirion RNA (plus strand) has been examined to see whether this sequence extends to the 5'-nucleotide terminus of the plus strand, or whether minus-strand synthesis terminates prematurely, perhaps due to the presence of a nonreplicated nucleotide primer for initiation of plus-strand synthesis. The 3' terminus was labeled with 32P using [5'-32P]pCp and RNA ligase, and complete RNase digests were performed with RNases A, T1, and U2. 32P-oligonucleotides were analyzed for size by polyacrylamide-urea gel electrophoresis. The major oligonucleotide products formed were consistent with the minus strand containing 3' ends complementary and flush with the 5' end of the plus strand. However, a variable proportion of the isolated minus strands from different preparations were heterogeneous in length and appeared to differ from each other by the presence of one, two, or three 3'-terminal A residues.
机译：已经检查了与酚硫鎓RNA（加链）互补的链（减去股线）的3'末端，以了解该序列是否延伸到加号的5'-核苷酸末端，或者是否减去束合成过早终止。由于存在非重杂化核苷酸引物，用于引发正链合成。使用[5'-32P] PCP和RNA连接酶，用32P标记3'末端，用RNASE A，T1和U2进行完整的RNase消化。通过聚丙烯酰胺 - 尿素凝胶电泳分析32P-寡核苷酸的尺寸。形成的主要寡核苷酸产物与含有3'末端的减号互补，与加号的5'末端齐平。然而，来自不同制剂的分离的减去股线的可变比例的长度是非均相的，并且通过一个，两个或三个3'-末端残留物而似乎彼此不同。

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