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首页> 外文期刊>Journal of Virology >Abelson murine leukemia virus transformation-defective mutants with impaired P120-associated protein kinase activity.
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Abelson murine leukemia virus transformation-defective mutants with impaired P120-associated protein kinase activity.

机译：Abelson鼠白血病病毒转化缺陷型突变体，具有P120相关蛋白激酶活性受损。

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Several transformation-defective (td) mutants of Abelson murine leukemia virus (AbLV) are described. Cells nonproductively infected with such mutants exhibited a high degree of growth contact inhibition, failed to form colonies in soft agar, lacked rescuable transforming virus, and were as susceptible as uninfected control cells to transformation by wild-type (wt) AbLV pseudotype virus. In addition, each of several td AbLV nonproductively infected cell clones analyzed was found to be nontumorigenic in vivo. Biochemical analysis of td mutant AbLV-infected clones revealed levels of expression of the major AbLV translational product, P120, and a highly related 80,000-Mr AbLV-encoded protein, P80, at concentrations analogous to those in wt AbLV-transformed cells. Although the AbLV-specific 120,000-Mr polyproteins expressed in td mutant AbLV-infected clones were indistinguishable from those in wt AbLV-transformed lines with respect to molecular weight and [35S]methionine tryptic peptide composition, they each differed from wt AbLV P120 in their patterns of post-translational phosphorylation. A previously described AbLV-associated protein kinase activity is shown to recognize as substrate a major tyrosine-specific acceptor site(s) contained within a single well-resolved tryptic peptide common to both AbLV P120 and P80. In vitro [gamma-32P]ATP-mediated labeling of this phosphorylation site was reduced to below detectable levels in td mutant nonproductively infected cell clones. These findings establish that the AbLV-encoded polyprotein P120 and its associated protein kinase activity are involved in AbLV tumorigenesis.
机译：描述了Abelson鼠白血病病毒（ABLV）的几种转化缺陷（Td）突变体。具有这种突变体的细胞未培养地感染的细胞表现出高度的生长接触抑制，未能在软琼脂中形成菌落，缺乏救援转化病毒，并且与未感染的对照细胞以野生型（WT）ABlv假型病毒转化为转化。此外，发现几种TD ABLV非产品感染的细胞克隆分析在体内Nontumorigenic。 TD突变体ABlv感染克隆的生化分析显示出主要ABLV平移产物，P120和高效的80,000-mr Ablv编码蛋白P80的表达水平，其浓度类似于WT Ablv转化细胞中的浓度。尽管在TD突变体Ablv感染的克隆中表达的特异性120,000mR多蛋白与分子量和[35s]甲硫氨酸胰蛋白肽组合物中的wt Ablv变化的线中的那些难以区分，但它们各自与其中的wt ablv p120不同翻译后磷酸化的图案。先前描述的Ablv相关的蛋白激酶活性显示为底物作为底物识别出包含在Ablv P120和P80共同的单个分离的胰蛋白酶肽内的主要酪氨酸特异性受体位点。在体外γ-32P] ATP介导的该磷酸化位点的标记降低至TD突变体非生产感染细胞克隆的可检测水平以下。这些发现确定了ABLV编码的多蛋白P120及其相关的蛋白激酶活性涉及ABLV肿瘤瘤。

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