Adenovirus-specific DNA-binding protein inhibits the hydrolysis of DNA by DNase in vitro.

K Nass; G D Frenkel

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Adenovirus-specific DNA-binding protein inhibits the hydrolysis of DNA by DNase in vitro.

机译：腺病毒特异性DNA结合蛋白通过体外通过DNase抑制DNA的水解。

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The adenovirus-specific DNA-binding protein was isolated from adenovirus type 5-infected KB cells and shown to possess DNase inhibitor activity. The protein decreased the rate of hydrolysis of single-strand DNA proportionately to its concentration in the reaction. Two peaks of activity were obtained upon sedimentation in a glycerol gradient, probably corresponding to the two major adenovirus-specific polypeptides in the preparation (molecular weights, 72,000 and 44,000). The DNase inhibitor activity of the adenovirus DNA-binding protein was distinguishable from that of the cellular DNA-binding protein, which we have described previously (K, Nass and G. D. Frenkel, J. Biol. Chem. 254:3407-3410, 1979), by its pattern of sedimentation and by the effect of temperature on the two activities. For the adenovirus DNA-binding protein, the ratio of DNase inhibitor activity at 43 degrees C to that at 30 degrees C was approximately 14, whereas for the cellular protein this ratio was less than 3. The DNase inhibitor activity with the temperature coefficient of 14 was absent from cells infected with adenovirus type 5 ts125 at 40 degrees C. DNase inhibition is a simple, sensitive, quantitative method for assay of the adenovirus DNA-binding protein.

机译：特异性腺病毒特异性DNA结合蛋白与腺病毒型5感染的KB细胞分离，并显示出具有DNase抑制剂活性。蛋白质在反应中成比例地降低了单链DNA的水解速率。在甘油梯度沉淀时获得了两种活性峰，可能对应于制剂（分子量，72,000和44,000）中的两个主要腺病毒特异性多肽。腺病毒DNA结合蛋白的DNase抑制剂活性与我们先前描述的细胞DNA结合蛋白的DNA酶抑制剂活性（K，NASS和GD Frenkel，J.Biol.Chem.254：3407-3410,1979），通过其沉积模式和温度对两种活动的影响。对于腺病毒DNA结合蛋白，DNA酶抑制剂活性在43℃至30℃下的比例约为14，而对于细胞蛋白，该比例小于3. DNA酶抑制剂活性为14的温度系数在40摄氏度下感染腺病毒型5 TS125的细胞不存在。DNase抑制是一种简单，灵敏，定量的方法，用于腺病毒DNA结合蛋白的测定。

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《Journal of Virology》 |1980年第2期|共6页
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K Nass; G D Frenkel;
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收录信息美国《科学引文索引》(SCI);
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1. Adenovirus-specific DNA-binding protein inhibits the hydrolysis of DNA by DNase in vitro. [J] . K Nass, G D Frenkel Journal of Virology . 1980,第2期

机译：腺病毒特异性DNA结合蛋白通过体外通过DNase抑制DNA的水解。
2. Adenovirus-specific DNA-binding protein inhibits the hydrolysis of DNA by DNase in vitro. [J] . K Nass, G D Frenkel Journal of Virology . 1980,第2期

机译：腺病毒特异性DNA结合蛋白通过体外通过DNase抑制DNA的水解。
3. Chromatin structure at the flanking regions of the human beta-globin locus control region DNase I hypersensitive site-2: proposed nucleosome positioning by DNA-binding proteins including GATA-1 [J] . Davies N, Freebody J, Murray V Biochimica et biophysica acta. Gene structure and expression . 2004,第3期

机译：人类β球蛋白基因座控制区DNase I超敏位点2侧翼区域的染色质结构：建议的DNA结合蛋白（包括GATA-1）对核小体的定位
4. USING SINGLE MOLECULAR PROBES TO DETECT DNA AND DNA-BINDING PROTEINS [C] . Giovanni Zocchi Annual Conference Nanotech and Biotech Convergence 2004 vol.3; 20040328-30; Cambridge,MA(US) . 2004

机译：使用单个分子检测DNA和DNA结合蛋白
5. THE RESTRICTION ENDONUCLEASE ECO RI: ITS INTRINSIC METAL CENTER AND SPECIFIC INTERACTIONS WITH A DNA RECOGNITION SITE (PROTEIN-NUCLEIC ACID, ZINC METALLOENZYMES, INTERACTIONS CHIRAL METAL COMPLEXES, DNA-BINDING PROTEINS). [D] . PARANAWITHANA, SHANTHI R. 1986

机译：限制性内切酶Eco RI：其内在金属中心以及与DNA识别位点的特定相互作用（蛋白-核酸，锌金属酶，相互作用的手性金属络合物，DNA结合蛋白）。
6. Adenovirus-specific DNA-binding protein inhibits the hydrolysis of DNA by DNase in vitro. [O] . K Nass, G D Frenkel 1980

机译：腺病毒特异性DNA结合蛋白在体外可抑制DNase水解DNA。
7. Adenovirus-specific DNA-binding protein inhibits the hydrolysis of DNA by DNase in vitro [O] . K Nass, G D Frenkel 1980

机译：腺病毒特异性DNA结合蛋白通过体外DNase抑制DNA的水解

Adenovirus-specific DNA-binding protein inhibits the hydrolysis of DNA by DNase in vitro.

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