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首页> 外文期刊>Journal of Virology >Separation of cyanogen bromide-cleaved peptides of the vesicular stomatitis virus glycoprotein and analysis of their carbohydrate content.
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Separation of cyanogen bromide-cleaved peptides of the vesicular stomatitis virus glycoprotein and analysis of their carbohydrate content.

机译：囊泡口炎病毒糖蛋白的氰基溴化肽分离及其碳水化合物含量分析。

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摘要

The purified glycoprotein of vesicular stomatitis virus was cleaved at methionine residues with cyanogen bromide, and the resultant peptides were analyzed by two-dimensional electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. Five peptide bands were resolved in cylindrical gels run under nonreducing conditions. After reduction and electrophoresis in the second dimension, 11 peptides were resolved, indicating that several were originally linked by disulfide bonds. Double-label experiments indicated that at least 8 of the 11 peptides were unique. The major oligosaccharide chains were attached to two different cyanogen bromide peptides. In addition, six other peptides contained small amounts of sialic acid, fucose, and mannose, indicating that the glycoprotein contains more carbohydrate chains than the two major ones which have been reported previously.
机译：用溴化亚氰基氨基乙硫氨酸残留物在甲硫氨酸残基中裂解纯化的糖蛋白，并通过二烷基硫酸钠 - 聚丙烯酰胺凝胶中的二维电泳分析所得肽。在圆柱形凝胶中拆分五个肽带在未添加的条件下运行。在第二尺寸减少和电泳后，分解11个肽，表明几种最初用二硫键连接。双标签实验表明，11种肽中的至少8个是独特的。主要的寡糖链附着在两种不同的氰基溴化酯上。此外，六种其他肽含有少量的唾液酸，岩藻糖和甘露糖，表明糖蛋白含有比以前报道的两个主要的碳水化合物链。

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