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首页> 外文期刊>Nucleic acids research >Identification and partial characterization of an endogenous form of mouse mammary tumor virus that is transcribed into the virion-associated RNA genome
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Identification and partial characterization of an endogenous form of mouse mammary tumor virus that is transcribed into the virion-associated RNA genome

机译：鉴定和部分表征在病毒性相关RNA基因组中转录的小鼠乳腺肿瘤病毒的内源形式

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Restriction mapping demonstrated the presence of several distinct proviral forms of mouse mammary tumor virus in the genome of GR mice. One of these proviruses (GR-MTV-2) was highly amplified in GR 3A cells, a cell line derived from a GR mammary tumor. By the criterion of restriction mapping, the amplified GR-MTV-2 provirus found in GR 3A cells was identical to the provirus found in Ml.19 cells, a rat cell line infected with virions obtained from GR 3A culture fluid. This result clearly implies that the GR-MTV-2 provirus in GR 3A cells was transcribed into the virion-associated viral RNA genome. Cleavage of either GR 3A or Ml.19 cell DNAs with the restriction enzyme Bgl II gave rise to a 2.6×106 dalton GR-MTV-2 proviral fragment (ca. 45% of the viral genome). This fragment was isolated and mapped with thirteen restriction enzymes.
机译：限制性映射证明了GR小鼠基因组中的几种不同的透过透过的小鼠乳腺肿瘤病毒。这些潜水术（GR-MTV-2）中的一种在GR 3A细胞中高度扩增，该细胞系来自GR乳腺肿瘤。通过限制映射的标准，在GR 3A细胞中发现的扩增GR-MTV-2潜水员与ML.19细胞中发现的潜水艇相同，一种感染从GR 3A培养液获得的病毒粒子的大鼠细胞系。该结果清楚地意味着GR 3A细胞中的GR-MTV-2潜意术被转录到病毒虫相关病毒RNA基因组中。具有限制酶BGL II的GR 3A或ML.19细胞DNA的切割产生2.6×10 6 Dalton GR-MTV-2透过碎片（约45％的病毒基因组） 。将该片段分离并用十三个限制酶映射。

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