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首页> 外文期刊>Journal of Virology >Partial amino-terminal sequences of the polyoma nonhistone proteins VP1, VP2, and VP3 synthesized in vitro.
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Partial amino-terminal sequences of the polyoma nonhistone proteins VP1, VP2, and VP3 synthesized in vitro.

机译:多瘤非甾酮蛋白VP1,VP2和VP3的局部氨基末端序列在体外合成。

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The three polyoma virus capsid proteins VP1, VP2, and VP3 were synthesized in vitro in the presence of several radiolabeled amino acids and, after purification on sodium dodecyl sulfate-polyacrylamide gels, were subjected to sequential Edman degradation. The partial amino-terminal amino acid sequences obtained were compared with the sequence of amino acids predicted from the polyoma virus DNA sequencing (Arrand et al., J. Virol. 33:606--618, 1980). Together, these results showed that the 5' ends of the VP1, VP2, and VP3 coding sequences are located 1,217, 289, and 634 nucleotides, respectively, from the junction of HpaII restriction fragments 3 and 5.
机译:在几种放射性标记的氨基酸存在下在体外合成三种多瘤病毒衣壳蛋白VP1,VP2和VP3,并在纯化对十二烷基硫酸钠 - 聚丙烯酰胺凝胶后,进行连续的Edman降解。将得到的部分氨基末端氨基酸序列与从多瘤病毒DNA测序预测的氨基酸序列进行比较(Arrand等,J.Virol。33:606--618,1980)。这些结果表明,VP1,VP2和VP3编码序列的5'末端分别从HPAII限制片段3和5的结分别位于1,217,289和634个核苷酸。

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