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Early RNAs in SP82- and SP01-infected Bacillus subtilis may be processed.

机译:可以处理SP82-和SP01感染的枯草芽孢杆菌中的早期RNA。

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摘要

Transcription of SP82 and SP01 DNAs in vitro by Bacillus subtilis RNA polymerase yielded mostly large RNA species, with many in excess of 1,500 bases in length, whereas most of the RNAs synthesized in vivo early in infection were much smaller. Addition of an extract from uninfected B. subtilis to reaction mixtures containing RNAs synthesized in vitro generated additional discrete RNAs whose mobilities on polyacrylamide gels matched the mobilities of some of the smaller RNAs synthesized in vivo.
机译:通过枯草芽孢杆菌RNA聚合酶对SP82和SP01 DNA的转录产生大多数大的RNA物种,其中许多长度超过1,500个碱基,而在感染中的体内合成的大多数RNA也较小。从未感染的B.枯草芽孢杆菌加入亚芽孢杆菌以含有RNA的反应混合物,其在体外产生的额外的离散RNA,其在聚丙烯酰胺凝胶上的迁移率与体内合成的一些较小RNA的迁移率匹配。

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