Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield

E G Afting; M L Recker

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Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield

机译：具有高产的两步亲和力 - 色谱纯度从猪肌瘤纯化

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pCathepsin D was purified by two-step affinity chromatography on concanavalin A- and pepstatin-Sepharose. The main purification was achieved by washing the enzyme bound to the pepstatin-Sepharose column with buffered 6 M-urea. This step separated cathepsin D from all low- and high-molecular-weight impurities. Although the 1700-fold purified acid proteinase was homogeneous on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, it still showed microheterogeneity./p

机译：通过两步亲和力色谱法在Concanavalin A-和胃蛋白酶 - Sepharose上纯化组织蛋白酶D.通过用缓冲的6m-urea洗涤与胃抑素 - 琼脂糖柱结合的酶来实现主要纯化。该步骤从所有低分子量和高分子量杂质中分离了组织蛋白酶D.虽然1700倍纯化的酸蛋白酶在十二烷基硫酸钠/聚丙烯酰胺 - 凝胶电泳上均匀，但它仍然显示出微渗透性。

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《The biochemical journal》 |1981年第2期|共页
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E G Afting; M L Recker;
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收录信息美国《科学引文索引》(SCI);
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1. Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield [J] . E G Afting, M L Recker The biochemical journal . 1981,第2期

机译：具有高产的两步亲和力 - 色谱纯度从猪肌瘤纯化
2. Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield [J] . E G Afting, M L Recker The biochemical journal . 1981,第2期

机译：具有高产的两步亲和力 - 色谱纯度从猪肌瘤纯化
3. Cathepsin D from pig myometrium. Characterization of the proteinase [J] . E G Afting, R Barth The biochemical journal . 1984,第3期

机译：猪子宫肌层组织蛋白酶D。蛋白酶的表征
4. Influences of pig breed and slaughter age on cathepsin B+L activity in fresh meat and dry-cured hams [C] . Tessema B., Storrustloken L., Alvseike O., ICoMST 2011;International conference of meat science and technology . 2012

机译：猪的品种和屠宰年龄对鲜肉和干腌火腿中组织蛋白酶B + L活性的影响
5. Expression, purification, and characterization of the mast cell proteases chymase and cathepsin G. [D] . Lockhart, Brent. 2008

机译：肥大细胞蛋白酶糜蛋白酶和组织蛋白酶G的表达，纯化和鉴定。
6. Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield. [O] . E G Afting, M L Recker 1981

机译：两步亲和色谱法从猪子宫肌层组织蛋白酶D的高收率纯化。
7. Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield. [O] . Afting, E G, Recker, M L 1981

机译：两步亲和色谱法从猪子宫肌层组织蛋白酶D的高收率纯化。
8. Purification and Properties of the Cathepsin D Type Proteinase from Beef and Rabbit Lung and Its Identification in Macrophages. [R] . Rojas-Espinosa, O., Dannenberg, A. M., Murphy, P. A., 1973

机译：牛和兔肺组织蛋白酶D型蛋白酶的纯化和性质及其在巨噬细胞中的鉴定。

Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield

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