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首页> 外文期刊>Journal of Virology >Detection and characterization of agarose-binding, capsid-like particles produced during assembly of a bacteriophage T7 procapsid.
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Detection and characterization of agarose-binding, capsid-like particles produced during assembly of a bacteriophage T7 procapsid.

机译：在组装噬菌体T7假膜组装期间产生的琼脂糖结合，衣壳状颗粒的检测和表征。

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摘要

It has previously been shown that: (i) during infection of its host, the DNA bacteriophage T7 assembles a DNA-free procapsid (capsid I), a capsid with an envelope differing physically and chemically from the capsid of the mature bacteriophage, and (ii) capsid I converts to a capsid (capsid II) with a bacteriophage-like envelope as it packages DNA. Lysates of phage T7-infected Escherichia coli contained a particle (AG particle) which copurified with capsid II during buoyant density sedimentation, velocity sedimentation, and solid support-free electrophoresis, but was distinguished from capsid II by its apparent diversity during electrophoresis in agarose gels. Treatment of AG particles with trypsin converted most of them to particles that comigrated with trypsin-treated capsid II during electrophoresis in agarose gels. Irreversible binding of AG particles to agarose gels was shown to contribute to the apparent diversity of AG particles during agarose gel electrophoresis. The results of quantitation of AG particles and of capsid I and capsid II in lysates of a nonpermissive host infected with T7 amber mutants suggested that, in site of their capsid II-like properties, most AG particles were produced during assembly of capsid I and not during DNA packaging. The presence of AG particles in T7 lysates explains contradictions in previous data concerning the pathway of T7 assembly.
机译：它先前已经显示：（i）在感染其宿主期间，DNA噬菌体T7组装了一种无DNA的假冒胶囊（衣壳I），衣壳的囊囊与成熟噬菌体的衣壳物理和化学不同，以及（ II）衣壳I转化为衣壳（Capsid II），含有噬菌体样包膜，因为它包装DNA。噬菌体T7感染的大肠杆菌的裂解物含有粒子（Ag颗粒），其在浮便密度沉降，速度沉降和固体支持电泳期间用衣壳II用胶囊II进行，但在琼脂糖凝胶中的电泳期间通过其表观多样性与衣壳II的显而易识。用胰蛋白酶处理Ag颗粒将大部分转化为在琼脂糖凝胶电泳期间用胰蛋白酶处理的衣壳II进行的颗粒。 Ag颗粒对琼脂糖凝胶的不可逆结合显示出琼脂糖凝胶电泳期间Ag颗粒的表观多样性。在感染T7琥珀色突变体的非发病宿主的裂解物中定量Ag颗粒和衣壳I和衣壳II的结果表明，在其衣壳II样性的位点，大多数Ag颗粒在组装衣壳I而不是在DNA包装期间。 T7裂解物中Ag颗粒的存在解释了关于T7组件通路的先前数据中的矛盾。

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