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首页> 外文期刊>Journal of Virology >Induction of Epstein-Barr virus nuclear antigen and DNA synthesis in a human epithelial cell line after Epstein-Barr virus infection.
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Induction of Epstein-Barr virus nuclear antigen and DNA synthesis in a human epithelial cell line after Epstein-Barr virus infection.

机译：Epstein-Barr病毒感染后人上皮细胞系中Epstein-Barr病毒核抗原和DNA合成的诱导。

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The association of Epstein-Barr virus (EBV) with nasopharyngeal carcinoma is supported by the presence of EBV genomes in the epithelial elements of the tumor and by elevated antibody titers to EBV-specific antigens in the patients; the levels of these titers are related to the clinical course of the disease. However, since most laboratory data suggest that EBV is a B-lymphotropic virus, it is unclear how the virus becomes associated with the epithelial elements of the nasopharynx. The purpose of the present work was to find a human model system to study this association. A human epithelial line (U) was found that could be directly infected by EBV, and viral functions, the induction of EBV nuclear antigen and cellular DNA synthesis, were demonstrated. The U line was established in 1957 by the late H. J. Van Kooten (Kok-Doorschodt at the University of Utrecht), and although it is no longer diploid, it exhibits density inhibition. When U cells were infected with EBV, EBV nuclear antigen was expressed in 6 to 16% of the cells, 1 and 2 days after infection with B95-8 virus, but not with the P3HR-1 strain. No evidence for virus replication was obtained; immunofluorescence staining for early antigens and virus capsid antigens gave negative results. Quantitative adsorption experiments for EBV indicated that the adsorption capacity of U cells is significant (60% of Raji cells). The present results also demonstrated that infection with the virus overcomes block(s) in cellular DNA synthesis caused by 5-fluorodeoxyuridine. The induction of DNA synthesis was determined by increased incorporation of [3H]thymidine into the cells. The highest level of isotope incorporation was observed at about 15 h after infection and thereafter decreased. Analysis of the induced DNA indicated that it was of cellular origin.
机译：通过肿瘤上皮元素中的EBV基因组和升高的抗体滴度与患者的EBV特异性抗原的升高的抗体滴度存在，支持与鼻咽癌的鼻咽癌的关联。这些滴度的水平与疾病的临床过程有关。然而，由于大多数实验室数据表明EBV是B淋巴细胞病毒，因此目前尚不清楚病毒如何与鼻咽的上皮元素相关。本工作的目的是找到一种研究这一协会的人类模型系统。发现人的上皮线（U）可以直接受到EBV感染，并且病毒功能，EBV核抗原和细胞DNA合成的诱导被证明。 U系列于1957年由H. J.Van Kooten（乌得勒支大学Kok-Doorschodt）成立，虽然它不再是二倍体，但它表现出密度抑制。当UBV感染U细胞时，EBV核抗原在用B95-8病毒感染后的6-16％的细胞中，1和2天表示，但不具有P3HR-1菌株。没有获得病毒复制的证据;早期抗原和病毒衣壳抗原的免疫荧光染色得到了负面结果。 EBV的定量吸附实验表明U细胞的吸附能力是显着的（Raji细胞的60％）。本结果还表明，对病毒感染克服了由5-氟脱氧尿苷引起的细胞DNA合成中的嵌段。通过将[3H]胸苷掺入细胞中的掺入，测定DNA合成的诱导。在感染后约15小时观察到最高水平的同位素掺入，然后减少。诱导的DNA分析表明它是细胞来源。

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