...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Adenovirus type 12-transformed rat embryo brain and rat liver epithelial cell lines: adenovirus type 12 genome content and viral protein expression.
【24h】

Adenovirus type 12-transformed rat embryo brain and rat liver epithelial cell lines: adenovirus type 12 genome content and viral protein expression.

机译：腺病毒型12转化大鼠胚胎脑和大鼠肝上皮细胞系：腺病毒型12基因组含量和病毒蛋白表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

By Southern blotting analysis, six adenovirus type 12 (Ad-12)-transformed rat embryo brain cell lines and one Ad-12-transformed rat liver epithelial line were shown to contain all or nearly all the Ad-12 genome. Another Ad-12 rat liver epithelial cell line contained a repeating structure consisting of only the left-hand 16% of the Ad-12 genome. Three Ad-12-specified proteins (molecular weights, 52,000, 41,000, and 18,000) were found by immunoprecipitation to be common to all of these cell lines. Two rat liver epithelial lines, produced from an Ad-12-infected culture and previously shown to be T-antigen negative by immunofluorescence, contained no detectable Ad-12 genome or Ad-12-specified proteins. Although some of the rat embryo brain transformants had been shown previously to express "late" Ad-12 mRNA, no Ad-12 structural proteins were found to be produced by these cell lines.
机译：通过Southern印迹分析，显示六种腺病毒型12（Ad-12） - 转换大鼠胚胎脑细胞系和一个Ad-12转化的大鼠肝上皮线，含有全部或几乎所有的Ad-12基因组。另一个Ad-12大鼠肝上皮细胞系包含仅由Ad-12基因组的左手16％组成的重复结构。通过免疫沉淀，发现三种Ad-12指定的蛋白质（分子量，52,000,41,000和18,000）普及所有这些细胞系。由Ad-12感染培养物产生的两条大鼠肝上皮线，并以免疫荧光所示为T-抗原，含有可检测的AD-12基因组或ad-12特定蛋白。尽管先前已经显示了一些鼠胚胎转化体以表达“晚期”Ad-12 mRNA，但没有发现这些细胞系产生的Ad-12结构蛋白。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号