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首页> 外文期刊>Journal of Virology >McDonough feline sarcoma virus: characterization of the molecularly cloned provirus and its feline oncogene (v-fms).
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McDonough feline sarcoma virus: characterization of the molecularly cloned provirus and its feline oncogene (v-fms).

机译：McDoNough猫科动物病毒：分子克隆的潜水症的表征及其猫鞘翅目（V-FMS）。

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The genetic structure of the McDonough strain of feline sarcoma virus (SM-FeSV) was deduced by analysis of molecularly cloned, transforming proviral DNA. The 8.2-kilobase pair SM-FeSV provirus is longer than those of other feline sarcoma viruses and contains a transforming gene (v-fms) flanked by sequences derived from feline leukemia virus. The order of genes with respect to viral RNA is 5'-gag-fms-env-3', in which the entire feline leukemia virus env gene and an almost complete gag sequence are represented. Transfection of NIH/3T3 cells with cloned SM-FeSV proviral DNA induced foci of morphologically transformed cells which expressed SM-FeSV gene products and contained rescuable sarcoma viral genomes. Cells transformed by viral infection or after transfection with cloned proviral DNA expressed the polyprotein (P170gag-fms) characteristic of the SM-FeSV strain. Two proteolytic cleavage products (P120fms and pp55gag) were also found in immunoprecipitates from metabolically labeled, transformed cells. An additional polypeptide, detected at comparatively low levels in SM-FeSV transformants, was indistinguishable in size and antigenicity from the envelope precursor (gPr85env) of feline leukemia virus. The complexity of the v-fms gene (3.1 +/- 0.3 kilobase pairs) is approximately twofold greater than the viral oncogene sequences (v-fes) of Snyder-Theilen and Gardner-Arnstein FeSV. By heteroduplex, restriction enzyme, and nucleic acid hybridization analyses, v-fms and v-fes sequences showed no detectable homology to one another. Radiolabeled DNA fragments representing portions of the two viral oncogenes hybridized to different EcoRI and HindIII fragments of normal cat cellular DNA. Cellular sequences related to v-fms (designated c-fms) were much more complex than c-fes and were distributed segmentally over more than 40 kilobase pairs in cat DNA. Comparative structural studies of the molecularly cloned proviruses of Synder-Theilen, Gardner-Arnstein, and SM-FeSV showed that a region of the feline-leukemia virus genome derived from the pol-env junction is represented adjacent to v-onc sequences in each FeSV strain and may have provided sequences preferred for recombination with cellular genes.
机译：通过分析分子克隆，转化荧光DNA的分析推导出猫赛素病毒（SM-Fesv）的遗传结构。 8.2千碱基对SM-FESV潜水员比其他猫群病毒的肝脏长，并且含有由源自猫白血病病毒的序列侧翼的转化基因（V-FMS）。关于病毒RNA的基因的顺序是5'-Gag-FMS-env-3'，其中表示整个猫的白血病病毒环境和几乎完整的GAG序列。用克隆的SM-FESV荧光DNA诱导NIH / 3T3细胞的转染与形态转化的细胞的焦点，其表达SM-FESV基因产物并含有救援的SARCOMA病毒基因组。通过病毒感染或用克隆的荧光DNA转染后转化细胞表达了SM-Fesv菌株的聚蛋白（P170GAG-FMS）特征。两种蛋白水解裂解产物（P120FMS和PP55GAG）也被从代谢标记的转化细胞中的免疫沉淀物中发现。在SM-FESV转化体中相对低地检测的另外的多肽在来自猫般的白血病病毒的包络前体（GPR85ENV）中难以区分的大小和抗原性。 V-FMS基因（3.1 +/- 0.3千碱基对）的复杂性大约比Snyder-Thilen和Gardner-Arnstein Fesv的病毒癌基因序列（V-FES）大致大致两倍。通过异络，限制酶和核酸杂交分析，V-FMS和V-FES序列彼此没有检测到的同源性。放射性标记的DNA片段代表两种病毒癌基因的部分与普通猫细胞DNA的不同EcoRI和HindIII片段杂交。与V-FMS（指定的C-FMS）相关的细胞序列比C-FES更复杂，并且在猫DNA中分段分段超过40千碱基对。 Synder-Thiilen，Gardner-Arnstein和SM-Fesv的分子克隆潜水物的比较结构研究表明，衍生自Pol-Env结的猫型白血病病毒基因组的区域在每个FESV中的V-ONC序列附近表示菌株并且可以提供优选与细胞基因重组的序列。

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