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首页> 外文期刊>Journal of Virology >Evidence for two classes of chromatin-associated Epstein-Barr virus-determined nuclear antigen.
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Evidence for two classes of chromatin-associated Epstein-Barr virus-determined nuclear antigen.

机译：两类染色质相关的Epstein-Barr病毒确定的核抗原的证据。

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A new class of Epstein-Barr virus nuclear antigen (EBNA) was identified by the complement fixation assay. This new species of EBNA is more tightly bound to chromatin and was termed class II EBNA, as opposed to the more weakly associated species, class I EBNA. Preparations of this new antigen(s) specifically reduced absorption with the titer of anti-EBNA antibodies as determined by the anticomplement immunofluorescence assay. Therefore, the complement fixation antigens (class II EBNA) appear to be related to the classical EBNA (class I EBNA). The class I EBNA was found to focus at the same pH (4.6) as the soluble antigen found in the cytosol. The class II EBNA differed from the class I EBNA with regard to its overall charge, molecular size, antigenicity, and affinity for chromatin. The class II EBNA appeared to be a basic protein, based on its apparent pI of 9.2 and its binding to cation-exchange resins. It differed from histones with regard to its molecular size (molecular weight between 60,000 and 70,000) and its elution from hydroxylapatite chromatography. Steps were taken to prevent proteolysis and artifacts in the immunological assays and in the overall charge estimation of the new antigen by nonspecific basic histone protein-acidic protein interactions. Both class I and class II EBNA were identified by radioimmunoelectrophoresis on two-dimensional polyacrylamide gels with pI values of 5.0 and 8.5, respectively, and a molecular weight range of 60,000 to 70,000 for both. A lower-molecular-weight antigen identified by molecular sieve chromatography appeared to be due to interference by histones in the immunoassays since it was not observed by the two-dimensional gel electrophoresis. Further characterization of this class II EBNA is in progress.
机译：通过补体固定测定鉴定了一种新的Epstein-Barr病毒核抗原（EBNA）。这种新的EBNA种类更紧密地与染色质更紧密，并且被称为II类EBNA，而不是更弱相关的物种，I类EBNA。这种新的抗原的制剂明确地降低了与抗EBNA抗体的滴度的吸收，如通过覆胸压免疫荧光测定法测定的。因此，补体固定抗原（II类EBNA）似乎与典型EBNA（I类EBNA）有关。发现I类EBNA以相同的pH（4.6）聚焦，因为在细胞溶胶中发现的可溶性抗原。 II类EBNA与IS EBNA不同，关于其总电荷，分子大小，抗原性和染色质的亲和力。 II类EBNA似乎是基于9.2的表观PI的基本蛋白质及其与阳离子交换树脂的结合。它与其分子大小（分子量在60,000和70,000之间）的不同之处不同，并且其从羟基磷灰石色谱中洗脱。采取步骤以防止免疫测定中的蛋白水解和伪影以及通过非特异性碱性组蛋白酸性蛋白质相互作用的新抗原的总电荷估计。 I类和II类EBNA都是通过在二维聚丙烯酰胺凝胶上的放射免疫电泳，PI值分别为5.0和8.5，分子量范围为60,000至70,000。通过分子筛色谱法鉴定的较低分子量抗原似乎是由于免疫测定中的组蛋白干扰，因为它未被二维凝胶电泳观察到。进一步表征该类II类EBNA正在进行中。

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