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首页> 外文期刊>Journal of Virology >Cloning of endogenous murine leukemia virus-related sequences from chromosomal DNA of BALB/c and AKR/J mice: identification of an env progenitor of AKR-247 mink cell focus-forming proviral DNA.
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Cloning of endogenous murine leukemia virus-related sequences from chromosomal DNA of BALB/c and AKR/J mice: identification of an env progenitor of AKR-247 mink cell focus-forming proviral DNA.

机译：来自BALB / C和AKR / J小鼠的染色体DNA的内源小鼠白血病病毒相关序列的克隆：鉴定AKR-247貂皮细胞聚焦形成荧光DNA的ENV祖细胞。

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Recombinant phages containing murine leukemia virus (MuLV)-reactive DNA sequences were isolated after screening of a BALB/c mouse embryo DNA library and from shotgun cloning of EcoRI-restricted AKR/J mouse liver DNA. Twelve different clones were isolated which contained incomplete MuLV proviral DNA sequences extending various distances from either the 5' or 3' long terminal repeat (LTR) into the viral genome. Restriction maps indicated that the endogenous MuLV DNAs were related to xenotropic MuLVs, but they shared several unique restriction sites among themselves which were not present in known MuLV proviral DNAs. Analyses of internal restriction fragments of the endogenous LTRs suggested the existence of at least two size classes, both of which were larger than the LTRs of known ecotropic, xenotropic, or mink cell focus-forming (MCF) MuLV proviruses. Five of the six cloned endogenous MuLV proviral DNAs which contained envelope (env) DNA sequences annealed to a xenotropic MuLV env-specific DNA probe; in addition, four of these five also hybridized to an ecotropic MuLV-specific env DNA probe. Cloned MCF 247 proviral DNA also contained such dual-reactive env sequences. One of the dual-reactive cloned endogenous MuLV DNAs contained an env region that was indistinguishable by AluI and HpaII digestion from the analogous segment in MCF 247 proviral DNA and may therefore represent a progenitor for the env gene of this recombinant MuLV. In addition, the endogenous MuLV DNAs were highly related by AluI cleavage to the Moloney MuLV provirus in the gag and pol regions.
机译：在筛选BALB / C小鼠胚胎DNA文库和EcoRI限制的AKR / J小鼠肝脏DNA的霰弹枪克隆后，分离含有鼠白血病病毒（Mulv） - 反应性DNA序列的重组噬菌体。分离了12个不同的克隆，其含有不完全的Mulv荧光DNA序列，从5'或3'长末端重复（LTR）延伸到病毒基因组中。限制性图表明内源性Mulv DNA与异胞外摩尔有关，但它们在其周期之间共享几个独特的限制性位点，其在已知的Mulv荧光DNA中不存在。内源性LTR的内部限制性片段的分析表明，存在至少两种尺寸的类别，这两种尺寸的均大于已知的生态转发剂，异孔或水貂细胞聚焦形成（MCF）Mulv潜水术的LTR。六个克隆内源Mulv玻璃玻璃DNA中的五种，其含有包络（ENV）DNA序列退火到异熵Mulv Env特异性DNA探针中;此外，这五种中的四种也与生态乳蛋白特异性env DNA探针杂交。克隆MCF 247荧光DNA还含有这种双反应性ENV序列。其中一种双反应性克隆的内源MULV DNA包含的env区域，其由alui和HPAII消化从MCF 247荧光DNA中的类似片段难以区分，因此可以代表该重组MULV的ENV基因的祖细胞。此外，内源性MULV DNA对GAG和POL区域的莫洛尼Mulv Provirus具有高度相关的玉米切割。

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