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首页> 外文期刊>Journal of Virology >Epstein-Barr Virus-Lymphoid Cell Interactions III. Effect of Concanavalin A and Saccharides on Epstein-Barr Virus Penetration
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Epstein-Barr Virus-Lymphoid Cell Interactions III. Effect of Concanavalin A and Saccharides on Epstein-Barr Virus Penetration

机译：Epstein-BARR病毒淋巴细胞相互作用III。康丹林A和糖类对Epstein-Barr病毒渗透的影响

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To study some aspects of Epstein-Barr virus (EBV) penetration into target cells, the effect of concanavalin A (ConA) and various saccharides on virus infectivity and cell susceptibility to EBV infection was examined. ConA treatment of the target cells, EBV, or EBV-cell complexes was found to inhibit virus antigen expression. Several control experiments with α-d-methyl-mannoside elution of ConA, removal of nonfused EBV particles from the cell surface by trypsin treatment, and addition of ConA at different times postinfection were performed to define the site of ConA action on EBV infection. ConA appeared to have a dual action: (i) it inhibited EBV binding to virus receptors, and (ii) it blocked the penetration of receptor-bound virus into target cells at a trypsin-sensitive stage, thus indicating that ConA prevented the fusion of viral envelope with the target cell membrane. A high sucrose concentration (0.25 M), known to inhibit cell membrane movements, was also found to block EBV penetration at a trypsinsensitive stage, thus suggesting the implication of cell membrane movements and underlying activities (or both) in viral envelope fusion. Lower concentrations of various monosaccharides (0.12 M) did not influence EBV infection. Under conditions of ConA treatment that did not influence EBV infectivity and target cells susceptibility, ConA was able to mediate virus binding to EBV receptornegative cell lines, but no virus antigens were expressed in these cells. These observations reinforced the idea that the mere attachment of EBV to lymphoid cells is not sufficient to lead to infection. In light of the present and previously published data, we postulate the existence of a specific cellular mechanism that allows the penetration of EBV into the target (B) lymphocyte.
机译：为了研究Epstein-Barr病毒（EBV）渗透到靶细胞中的一些方面，检查了康贾伐嗪A（Cona）和各种糖类对病毒感染性和对EBV感染的细胞敏感性的影响。发现靶细胞，EBV或EBV细胞复合物的Cona治疗抑制病毒抗原表达。几种对Cona的α-D-甲基 - 甘露糖苷洗脱的几种对照实验，通过胰蛋白酶处理除去来自细胞表面的非耐用的EBV颗粒，并进行了在不同时间的添加结论，以定义EBV感染的Cona作用位点。 Cona似乎具有双重作用：（i）它抑制EBV与病毒受体的结合，并且（ii）它阻止受体结合病毒在胰蛋白酶敏感阶段渗透到靶细胞中，因此表明Cona阻止了融合病毒包膜与靶细胞膜。还发现高蔗糖浓度（0.25μm）抑制细胞膜运动，以阻断胰蛋白酶溶液阶段的EBV渗透，从而表明细胞膜运动和基础活动（或两者）在病毒包膜融合中的含义。较低浓度的各种单糖（0.12米）不影响EBV感染。在不影响EBV感染性和靶细胞易感性的Cona治疗的条件下，Cona能够介导与EBV接收细胞系的病毒结合，但在这些细胞中没有表达病毒抗原。这些观察结果加强了EBV对淋巴细胞的附着不足以导致感染的想法。鉴于目前和先前公布的数据，我们假设存在特定细胞机制的存在，允许EBV渗透到靶（B）淋巴细胞中。

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