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首页> 外文期刊>Journal of Virology >Poliovirus-induced alterations in HeLa cell membrane functions.
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Poliovirus-induced alterations in HeLa cell membrane functions.

机译：Poliovirus诱导的HeLa细胞膜功能的改变。

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Protein synthesis, amino acid uptake, membrane potential, cell volume, Na+ and K+ levels, and ATPase (Na+,K+ activated; EC 3.6.1.3) activity were investigated in control and poliovirus-infected HeLa cells. Inhibition of protein synthesis was first observed 60 min postinfection and reached a maximum at 120 min. The onset of protein synthesis inhibition coincided with a decrease in cell volume and with an elevation of ATPase activity in isolated HeLa cell membranes. Some 3 h after virus adsorption, ATPase activity was inhibited, the Na+-K+ gradient of the cell collapsed, both membrane potential-dependent tetraphenylphosphonium ion uptake and amino acid uptake were reduced, and the cell volume increased. These results provide further experimental support for the hypothesis that modification of the cell membrane plays an important role in the strategy of cytopathogenic viruses in the shutoff of host metabolism and cell death.
机译：蛋白质合成，氨基酸摄取，膜电位，细胞体积，Na +和K +水平，以及ATP酶（Na +，K +活化; EC 3.6.1.3）活性，对照和脊髓灰质炎病毒感染的HeLa细胞进行活性。首先观察到蛋白质合成的抑制60分钟，并在120分钟内达到最大值。蛋白质合成抑制的发作与细胞体积的降低和升高的蛋白质活性升高，在分离的HeLa细胞膜中升高。在病毒吸附后的约3小时，抑制ATP酶活性，细胞的Na + -K +梯度塌陷，膜电位依赖性四苯基鏻离子吸收和氨基酸摄取，并且细胞体积增加。这些结果为该假设提供了进一步的实验支持，即细胞膜的修饰在宿主代谢和细胞死亡中在细胞瘤病毒的策略中起重要作用。

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