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首页> 外文期刊>Journal of Virology >Antibodies against a nonapeptide of polyomavirus middle T antigen: cross-reaction with a cellular protein(s).
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Antibodies against a nonapeptide of polyomavirus middle T antigen: cross-reaction with a cellular protein(s).

机译：针对多肽的抗体T抗原的抗体：与细胞蛋白质的交叉反应。

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Antibodies were raised against the sequence Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met -Glu, which represents a part of the middle T antigen of polyomavirus that is considered to be important in inducing the phenotype of transformed cells. The antibodies reacted with native as well as denatured middle T antigens. In addition, the antibodies immunoprecipitated a cellular protein with an apparent molecular weight of 130,000 (130K) from mouse and rat cells. In some cases, a 33K protein was also immunoprecipitated. Immunoprecipitation of middle T antigen as well as 130K and 33K proteins was blocked by the peptide. The antibodies labeled microfilaments of untransformed mouse, rat, human, and chicken cells by immunofluorescence. This labeling was also blocked by the peptide. The labeling pattern and distribution under a variety of conditions were indistinguishable from those of anti-actin antibodies, although no evidence has been obtained to indicate that the anti-peptide antibodies react with actin. The 130K protein migrated in sodium dodecyl sulfate-polyacrylamide gel electrophoresis slightly slower than chicken gizzard vinculin (130K) and slightly faster than myosin light-chain kinase of chicken smooth muscle (130K). Neither of these proteins absorbed the anti-peptide antibodies. The 33K protein does not seem to be tropomyosin (32K to 40K).
机译：针对序列Glu-glu-glu-glu-met-pro-met-glu升高的抗体，其代表多瘤的中间T抗原的一部分认为在诱导转化细胞的表型方面是重要的。抗体与天然和变性的中间T抗原反应。另外，抗体免疫沉淀的细胞蛋白，具有来自小鼠和大鼠细胞的表观分子量为130,000（130k）。在某些情况下，33k蛋白也被免疫沉淀。肽阻断中间T抗原以及130k和33K蛋白的免疫沉淀。该抗体通过免疫荧光标记未转化的小鼠，大鼠，人和鸡细胞的微丝。该标记也被肽堵塞。尽管未获得抗肌动蛋白抗体的那些难以区分的标记图案和分布，但没有得到证据表明抗肽抗体与actin反应。在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳中迁移的130K蛋白略微慢于鸡Gizzard Vinculin（130K），比鸡肉平滑肌的肌蛋白轻链激酶略微快，略微快速（130K）。这些蛋白质都没有吸收抗肽抗体。 33k蛋白似乎没有对原鸡（32K至40k）。

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