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首页> 外文期刊>Journal of Virology >Involvement of Escherichia coli K-12 DNA polymerase I in the growth of bacteriophage Mu.
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Involvement of Escherichia coli K-12 DNA polymerase I in the growth of bacteriophage Mu.

机译：大肠杆菌K-12 DNA聚合酶I参与噬菌体亩的生长。

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We examined several aspects of bacteriophage Mu development in Escherichia coli strains that carry mutations in the polA structural gene for DNA polymerase I (PolI). We found that polA mutants were markedly less efficient than PolI wild-type (PolI+) strains in their capacity to form stable Mu lysogens and to support normal lytic growth of phage Mu. The frequency of lysogenization was determined for polA mutants and their isogenic PolI+ derivatives, with the result that mutants were lysogenized 3 to 8 times less frequently than were PolI+ cells. In one-step growth experiments, we found that phage Mu grew less efficiently in polA cells than in PolI+ cells, as evidenced by a 50 to 100% increase in the latent period and a 20 to 40% decrease in mean burst size in mutant cells. A further difference noted in infected polA strains was a 10-fold reduction in the frequency of Mu-mediated transposition of chromosomal genes to an F plasmid. Pulse labeling and DNA-DNA hybridization assays to measure the rate of phage Mu DNA synthesis after the induction of thermosensitive prophages indicated that phage Mu replication began at about the same time in both polA and PolI+ strains, but proceeded at a slower rate in polA cells. We conclude that PolI is normally involved in the replication and integration of phage Mu. However, since phage Mu does not exhibit an absolute requirement for normal levels of PolI, it appears that residual PolI activity in the mutant strains, other cellular enzymes, or both can partially compensate for the absence of normal PolI activity.
机译：我们在大肠杆菌菌株中检​​查了噬菌体亩发育的几个方面，即在DNA聚合酶I（Poli）中携带Pola结构基因的突变。我们发现Pola突变体比Poli野生型（Poli +）菌株的能力显着较低，以形成稳定的Mu溶雾化，并支持噬菌体亩的正常毛细血管生长。为Pola突变体和其中源性Poli +衍生物测定溶酶化的频率，结果使突变体溶液溶液比Poli +细胞频率不太频繁。在一步的生长实验中，我们发现噬菌体在Poli +细胞中效率低于poli +细胞，如潜伏期的增加50％至100％，突变细胞中的平均爆破尺寸的20％〜40％降低。在感染的Pola菌株中注意到的进一步差异是在染色体基因对F质粒的频率转置的频率降低10倍。脉冲标记和DNA-DNA杂交测定测量噬菌体诱导后测量噬菌体MU DNA合成率，表明噬菌体μ复制在麦片和POLI +菌株中的约束时开始，但在POLA细胞中以较慢的速率进行。我们得出结论，POLI通常涉及噬菌体亩的复制和整合。然而，由于噬菌体MU没有表现出对正常水平的绝对要求，因此似乎突变菌株，其他细胞酶或两者中的残留poli活性可以部分地补偿不存在正常poli活性的情况。

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