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首页> 外文期刊>Journal of Virology >Emv-13 (Akv-3): a noninducible endogenous ecotropic provirus of AKR/J mice.
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Emv-13 (Akv-3): a noninducible endogenous ecotropic provirus of AKR/J mice.

机译：EMV-13（AKV-3）：AKR / J小鼠的非融业内源性生态缺乏。

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All AKR/J mice carry at least three endogenous ecotropic viral loci which have been designated Emv-11 (Akv-1), Emv-13 (Akv-3), and Emv-14 (Akv-4) (Jenkins et al., J. Virol. 43:26-36, 1982.) Using two independent AKR/J-derived sets of recombinant inbred mouse strains, AKXL (AKR/J x C57L/J) and AKXD (AKR/J x DBA/2J), as well as the HP/EiTy strain (an Emv-13-carrying inbred strain partially related to AKR/J mice) (Taylor et al., J. Virol. 23:106-109, 1977), we have examined the association of these endogenous viral loci with virus expression. Strains which transmit Emv-11 or Emv-14 or both were found to produce virus spontaneously, whereas strains that transmit Emv-13 alone were negative for virus expression. Restriction endonuclease digestion and hybridization with an ecotropic virus-specific hybridization probe of DNAs from strains which transmit only Emv-13 yielded enzyme cleavage patterns identical to those observed with DNAs from strains transmitting Emv-11 or Emv-14 or both. These findings indicate the absence of any gross rearrangement of Emv-13 proviral sequences. Cell cultures derived from recombinant inbred strains that carry only Emv-13 failed to express detectable infectious virus, viral proteins, or cytoplasmic ecotropic virus-specific RNA even after treatment with 5-iodo-2-deoxyuridine or 5-azacytidine, an inhibitor of DNA methylation. Our results indicate that a mechanism(s) other than methylation of Emv-13 proviral DNA is responsible for inhibition of Emv-13 expression.
机译：所有AKR / J小鼠携带至少三个内源的生态调节病毒基因座，已被指定为EMV-11（AKV-1），EMV-13（AKV-3）和EMV-14（AKV-4）（Jenkins等， J.Virol。43：26-36,1982。）使用两个独立的AKR / J衍生的重组近亲鼠标菌株，AKXL（AKR / J X C57L / J）和AKXD（AKR / J X DBA / 2J），以及HP / EITY菌株（携带部分与AKR / J小鼠的EMV-13携带的近铬菌株）（Taylor等人，J.Virol。23：106-109,1977），我们研究过协会这些内源性病毒位点与病毒表达。发现发射EMV-11或EMV-14或两者的菌株自发地产生病毒，而仅传播EMV-13的菌株仅为病毒表达为阴性。限制性内切核酸酶消化和杂交与来自菌株的DNA的生态病毒特异性杂交探针，其仅传递EMV-13产生的酶切割模式与从递送EMV-11或EMV-14或两者的菌株观察到的DNA观察的那些。这些发现表明没有任何溢出型荧光序列的任何总重排。衍生自重组近交菌株的细胞培养物，其仅携带EMV-13未能表达可检测的传染性病毒，病毒蛋白或细胞质的生态学病毒特异性RNA，甚至用5-碘-2-脱氧尿苷或5-氮杂扎含量，DNA抑制剂处理甲基化。我们的结果表明，除eMV-13荧光DNA的甲基化以外的机制是抑制EMV-13表达的原因。

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