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Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei

机译：四差异诱导诱导的基因：宏观和微核结构和组织

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The physical organization of eight Tetrahymena genes active during conjugation (meiosis) was examined in the somatic (macro-) and germinal (micro-) nuclei of this organism. Three of these genes make transcripts that are only detected during meiotic prophase. Southern blot analyses indicated that all genes examined were present in both nuclei. Except for one gene family (pC6), all appeared to be non-repetitive and there were no detectable sequence rearrangements in or near the genes. The exceptional gene was repeated approximately five to seven times and DNA rearrangement occurred in or near each of these copies. A comparison of cDNA and macro- and micronuclear DNA restrication maps indicated that one of the genes (cnj B) contains introns. This is the first report of evidence for introns in a non-ribosomal gene in Tetrahymena. A site specific modification probably due to adenine methylation was seen in the macronuclear copy of another gene (cnj C).
机译：在这种生物体的体细胞（宏观）和生发（微核）和生发（微核）中，检查八个四氢塞基因的物理组织在缀合（MeIosis）期间。这些基因中的三种使得仅在减数分裂预言期间检测到的转录物。 Southern印迹分析表明，检查的所有基因都存在于核中。除了一个基因家族（ p c6），似乎是非重复的，并且在基因中没有任何可检测的序列重排。卓越的基因大约重复约五到七次，并且在这些副本中的每一个中或接近每份的DNA重排。 cDNA和微核DNA限制图的比较表明，其中一种基因（CNJ B）含有内含子。这是四环塞中非核糖体基因内含子的第一证据报告。可能在另一基因（CNJ C）的常规核拷贝中观察到腺嘌呤甲基化的特异性修饰。

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