• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleic acids research >DNA replication is required for abundant expression of a plasmid-borne late US11 gene of herpes simplex virus type 1
【24h】

DNA replication is required for abundant expression of a plasmid-borne late US11 gene of herpes simplex virus type 1

机译：对于单纯疱疹病毒1型疱疹病毒的质粒源性晚期US11基因的丰富表达需要DNA复制

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

During herpes simplex virus type 1 (HSV-1) infection, the appearance of true-late gene products is severely reduced under conditions of DNA synthesis inhibition. This report describes the use of a plasmid-borne promoter of a true-late HSV-1 gene (US11), linked to the rabbit β-globin gene, to study the requirement of DNA replication for late gene expression. The activity of the plasmid-borne US11 promoter in constructs containing or lacking an HSV-1 origin of replication (ORIg) was analysed by quantitative SI mapping of correctly initiated hybrid transcripts. Following HSV-1 superinfection of transfected HeLa cells, the US11 promoter in ORI+ plasmids was expressed with similar kinetics to the viral US11 promoter. US11 promoter activity was first detected at the same time as the onset of DNA template replication. Expression of US11 RNA was detectable from non-replicating ORI- plasmids, although transcript accumulation was reduced by greater than 90%. Sequences containing the IE-5 promoter (a 3' co-terminal gene whose transcription starts 5' of US11) also played a positive role in achieving normal US11 gene expression.
机译：在单纯疱疹病毒1型（HSV-1）感染期间，在DNA合成抑制条件下严重降低了真末基因产物的外观。本报告描述了使用与兔β-珠蛋白基因相关的真正晚期HSV-1基因（US11）的质粒传播的启动子，研究DNA复制对晚期基因表达的要求。通过定量的Si映射分析含有或缺乏HSV-1的含HSV-1来源的构建体中的质粒的US11启动子的活性通过正确引发的杂交转录物进行分析。在转染的HeLa细胞的HSV-1超细感染之后，ORI + / sup>质粒的US11启动子以类似的动力学表达到病毒US11启动子。首先在DNA模板复制的发生同时检测US11启动子活性。虽然转录物积累降低了大于90％，但是从非复制矿石和质粒中可检测US11 RNA的表达。含有IE-5启动子的序列（转录US11的5'的3'末端基因）在实现正常的US11基因表达方面也发挥了积极作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号