Molecular cloning of a cDNA and assignment of the C-terminal of sarcotoxin IA, a potent antibacterial protein of Sarcophaga peregrina

H Komano; H Takahashi; M Okada; N Matsumoto; Q X Ming; S Natori; Y Nakajima; Y Nakanishi

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Molecular cloning of a cDNA and assignment of the C-terminal of sarcotoxin IA, a potent antibacterial protein of Sarcophaga peregrina

机译：CDNA的分子克隆和Sarcotoxin的C末端的分配是Sarcophaga Peregrina的有效抗菌蛋白质

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pA previous paper described the complete amino acid sequences of sarcotoxins IA, IB and IC, which are a group of potent antibacterial proteins with almost identical primary structures produced by Sarcophaga peregrina (fleshfly) larvae [Okada & Natori (1985) J. Biol. Chem. 260, 7174-7177]. The present paper describes the cDNA cloning and complete nucleotide sequencing of a cDNA clone for sarcotoxin IA. The C-terminal amino acid residue of sarcotoxin IA deduced from the nucleotide sequence was glycine, whereas it was found to be arginine by amino acid sequencing of purified sarcotoxin IA. Analysis of the elution profiles on h.p.l.c. of the synthetic derivatives of sarcotoxin IA showed that the C-terminal amino acid residue of authentic sarcotoxin IA is amidated arginine, which is probably produced by enzymic cleavage of terminal glycine./p

机译：>前论文描述了Sarcotoxins Ia，Ib和Ic的完整氨基酸序列，其是一组有效的抗菌蛋白，其具有几乎相同的主要结构，由Sarcophaga Peregrina（Fleshfly）幼虫（冈田＆amp; Natori（1985）J. Biol。化学。 260,7174-7177]。本文描述了CDNA克隆的cDNA克隆和完全核苷酸测序，用于Sarcotoxin Ia。从核苷酸序列推导的Sarcotoxin Ia的C末端氨基酸残基是甘氨酸，而发现它是通过纯化的SarcoToxin Ia的氨基酸测序的精氨酸。 H.P.L.C的洗脱曲线分析。 Sarcotoxin Ia的合成衍生物表明，正宗Sarcotoxin Ia的C-末端氨基酸残基是酰胺化精氨酸，其可能通过末端甘氨酸的酶切割产生。

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《The biochemical journal》 |1986年第3期|共页
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H Komano; H Takahashi; M Okada; N Matsumoto; Q X Ming; S Natori; Y Nakajima; Y Nakanishi;
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1. Molecular cloning of a cDNA and assignment of the C-terminal of sarcotoxin IA, a potent antibacterial protein of Sarcophaga peregrina [J] . H Komano, H Takahashi, M Okada, The biochemical journal . 1986,第3期

机译：CDNA的分子克隆和Sarcotoxin的C末端的分配是Sarcophaga Peregrina的有效抗菌蛋白质
2. Molecular cloning of a cDNA and assignment of the C-terminal of sarcotoxin IA, a potent antibacterial protein of Sarcophaga peregrina [J] . H Komano, H Takahashi, M Okada, The biochemical journal . 1986,第3期

机译：CDNA的分子克隆和Sarcotoxin的C末端的分配是Sarcophaga Peregrina的有效抗菌蛋白质
3. Analysis of a gene cluster for sarcotoxin II, a group of antibacterial proteins of Sarcophaga peregrina. [J] . A Kanai, S Natori Molecular and Cellular Biology . 1990,第12期

机译：sarcotoxin II（一组石棺Peregrina的抗菌蛋白）基因簇的分析。
4. Cloning of Genes Conferring Allergenic Proteins and Salt Tolerance by Screening a Full Length cDNA Library in Soybean [C] . Yuelin Zhu, Lifei Yang, Junyi Gai International Horticulture Congress on Science and Horticulture for People . 2012

机译：通过在大豆中筛选全长cDNA文库赋予过敏蛋白和耐盐性基因的克隆
5. Molecular study of diapause via generation of two cDNA libraries and partial cloning of the ecdysone receptor in Sarcophaga crassipalpis [D] . Ball, Elizabeth Nicole 2002

机译：通过产生两个cDNA文库和部分克隆蜕皮虫中蜕皮激素受体的滞育分子研究
6. Molecular cloning of a cDNA and assignment of the C-terminal of sarcotoxin IA a potent antibacterial protein of Sarcophaga peregrina. [O] . N Matsumoto, M Okada, H Takahashi, 1986

机译：cDNA的分子克隆和石蜡毒素IS（Sarcophaga peregrina的有效抗菌蛋白）的C末端分配。
7. Molecular cloning of a cDNA and assignment of the C-terminal of sarcotoxin IA, a potent antibacterial protein of Sarcophaga peregrina. [O] . Matsumoto, N, Okada, M, Takahashi, H, 1986

机译：cDNA的分子克隆和石蜡毒素IS（Sarcophaga peregrina的有效抗菌蛋白）的C末端分配。
8. Biomolecular Mechanism, Cloning, Sequencing and Analysis of Adaptive Reflection cDNAs and Proteins from Squid [R] . Morse, D. E. 2010

机译：鱿鱼适应性反射cDNa和蛋白质的生物分子机制，克隆，测序及分析

Molecular cloning of a cDNA and assignment of the C-terminal of sarcotoxin IA, a potent antibacterial protein of Sarcophaga peregrina

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