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首页> 外文会议>International Horticulture Congress on Science and Horticulture for People >Cloning of Genes Conferring Allergenic Proteins and Salt Tolerance by Screening a Full Length cDNA Library in Soybean
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Cloning of Genes Conferring Allergenic Proteins and Salt Tolerance by Screening a Full Length cDNA Library in Soybean

机译：通过在大豆中筛选全长cDNA文库赋予过敏蛋白和耐盐性基因的克隆

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摘要

To obtain genes conferring allergenic proteins and salt tolerance of vegetable soybean (Glycine max (L.) Merr.), a full-length cDNA library from the developing seeds of 'NN99-10' was constructed and characterized. Gel-electrophoresis results showed that most of the cDNA inserts ranged from 0.40 to 2.0 kb, with an average size c. 800 bp. Randomly-picked clones showed that the recombination rate was >99%. This indicated that the cDNA library constructed was a full-length library with high quality. After blue/white selection followed by sequencing of the positive cDNA clones from the constructed library, a total of 642 expressed sequence tags (ESTs) were identified, 468 of which contained full-length coding regions. Based on bioinformatic analysis of the sequences, a total of 395 full-length cDNA clones were homologous to various known genes in soybean and other species. The remaining 73 full-length cDNA clones showed little homology to known sequences, which might be considered novel. The identified 395 full-length cDNA clones fell into 8 functional categories, including modifications and precursors of protein (26.31%), protein synthesis (22.24%), and defense proteins (22.20%). A total of eleven genes of agronomic importance were identified, among which eight related to seed quality (four genes conferring allergenic proteins) and three conferring salt tolerance.
机译：为了获得赋予植物大豆的过敏蛋白和耐盐性（甘氨酸Max（L.）Merr的耐盐性的基因。构建和表征来自“NN99-10”的显影种子的全长cDNA文库。凝胶电泳结果表明，大多数cDNA插入物的范围为0.40至2.0kb，平均尺寸C。 800 bp。随机挑选的克隆显示重组率> 99％。这表明构建的cDNA文库是具有高质量的全长文库。在蓝色/白色选择之后，通过构造文库测序阳性cDNA克隆，鉴定了总共642个表达序列标签（EST），其中468个包含全长编码区。基于序列的生物信息分析，总共395个全长cDNA克隆对大豆和其他物种的各种已知基因同源。剩余的73个全长cDNA克隆显示出与已知序列的同源性很少，这可能被认为是新颖的。确定的395全长cDNA克隆落入8个功能类别，包括蛋白质的修饰和前体（26.31％），蛋白质合成（22.24％）和防御蛋白质（22.20％）。鉴定了总共11个基因，其中八个与种子质量（赋予过敏蛋白的四个基因）和三种赋予耐盐性有关。

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