Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus.

J Resnick; T Shenk

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Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus.

机译：Simian病毒40酰蛋白促进了主要衣壳多肽的正常核位置和病毒的细胞 - 细胞传播。

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The simian virus 40 agnoprotein is a 61-amino-acid, highly basic polypeptide that is coded within the 5' leader of late 16S mRNAs. To better understand agnoprotein function and to more effectively differentiate cis-from trans-acting effects of an agnogene mutation, we constructed a mutant virus that carries a single-base-pair substitution and fails to produce agnoprotein. pm 1493 contains a T/A to A/T transversion at sequence position 335. This mutation converts the agnoprotein initiation codon from ATG to TTG, preventing synthesis of the protein. The mutant displays only a modest growth defect in CV-1P and AGMK cells and no defect in BSC-1 cells. Early-gene expression, DNA replication, synthesis of late viral products, and the kinetics of virion assembly all appear normal in pm 1493-infected CV-1P cells. Immunofluorescent studies, however, indicate that localization of the major capsid polypeptide VP1 is different in mutant- than wild-type virus-infected cells. Furthermore, the lack of agnoprotein led to inefficient release of mature virus from the infected cell. Agnogene mutants could be severely compromised in their ability to propagate in monkeys given their reduced capacity for cell-to-cell spread.

机译：Simian病毒40酰胺蛋白是61-氨基酸，高度基本的多肽，其在16S后的5'MRNA的5'领导中编码。为了更好地了解酰胺蛋白功能并更有效地区分CIS-从酰基突变的反式作用效果，我们构建了一种突变病毒，其携带单碱基对替代物，并且不能产生酰蛋白。 PM 1493含有序列位置335的T / A至A / T频转向。该突变将酰蛋白引发密码子转化为ATG至TTG，防止蛋白质的合成。突变体仅在CV-1P和AGMK细胞中仅显示适度的生长缺陷，并且BSC-1细胞中没有缺陷。早期基因表达，DNA复制，晚期病毒产品的合成，以及病毒群组件的动力学在PM 1493感染的CV-1P细胞中显示出正常。然而，免疫荧光研究表明，主要胶囊多肽VP1的定位在突变体中不同于野生型病毒感染细胞。此外，缺乏酰胺蛋白导致来自感染细胞的成熟病毒的效率低下。酰胺突变体可能严重损害，因为它们在猴子中繁殖的能力，鉴于其降低的细胞对细胞扩散的能力。

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《Journal of Virology》 |1986年第3期|共9页
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J Resnick; T Shenk;
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收录信息美国《科学引文索引》(SCI);
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1. Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus. [J] . J Resnick, T Shenk Journal of Virology . 1986,第3期

机译：Simian病毒40酰蛋白促进了主要衣壳多肽的正常核位置和病毒的细胞 - 细胞传播。
2. Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus. [J] . J Resnick, T Shenk Journal of Virology . 1986,第3期

机译：Simian病毒40酰蛋白促进了主要衣壳多肽的正常核位置和病毒的细胞 - 细胞传播。
3. Simian virus 40 agnoprotein facilitates perinuclear-nuclear localization of VP1, the major capsid protein. [J] . S Carswell, J C Alwine Journal of Virology . 1986,第3期

机译：Simian病毒40酰蛋白促进了VP1的Perinualtar核定位，主要衣壳蛋白。
4. CHARACTERIZATION OF THE NUCLEAR MATRIX PREPARED FROM CELLS INFECTED WITH SIMIAN VIRUS 40 (REPLICATION). [D] . JONES, CLINTON JIMMIE. 1985

机译：从感染猿猴病毒40的细胞中制备的核基质的特性（复制）。
5. Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus. [O] . J Resnick, T Shenk 1986

机译：猿猴病毒40 agnoprotein促进主要衣壳多肽的正常核定位和病毒在细胞间的传播。
6. Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus [O] . J Resnick, T Shenk 1986

机译：Simian病毒40酰胺蛋白促进了主要衣壳多肽的正常核位置和病毒的细胞 - 细胞传播

Simian virus 40 agnoprotein facilitates normal nuclear location of the major capsid polypeptide and cell-to-cell spread of virus.

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