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首页> 外文期刊>Nucleic acids research >A single tRNA (guanine)-methyltransferase from Tetrahymena with both mono- and di-methylating activity
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A single tRNA (guanine)-methyltransferase from Tetrahymena with both mono- and di-methylating activity

机译：单个TRNA（鸟嘌呤） - 与单甲基甲酰化活性的四甲基转移酶

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摘要

A tRNA (guanine-2) methyltransferase has been purified to homogeneity from the protozoan Tetrahvmena pyriformis. The enzyme methylates purified E. coli tRNAs which have a guanine residue at position 26 from the 5′ end; it also methylates tRNA prepared from the m2 2G? yeast mutant trm 1. This methyltransferase is therefore equivalent to the guanine methyltransferase 2mGII found in mammalian extracts. The purified 2mGII from Tetrahvmena is capable of forming both N2 -methylguanine and N2 2-dimethylguanine on a single tRNA isoaccepting species; under conditions of limiting tRNA or long reaction times the predominant product is dimethylguanine. Analysis of the products formed under varying reaction conditions suggests that dimethylguanine formation is a two step process requiring dissociation of the enzyme-monomethylated tRNA intermediate.
机译：将TRNA（鸟嘌呤-2）甲基转移酶从原生动物Tetrahvmena Pyriformis纯化成均匀性。酶甲酸盐纯化的大肠杆菌TrNA，其在5'末端的26位具有鸟嘌呤残基;它还从M 2℃制备的TRNA甲基化物甲基化物质2 2℃ g β-酵母突变体Trm 1.因此，该甲基转移酶等同于发现的鸟嘌呤甲基转移酶2mgii哺乳动物提取物。来自Tetrahvmena的纯化的2MgII能够在单个TRNA IsoAccepting物种上形成N 2 -Methynine和N 2 -dimethyline;在限制TRNA或长反应时间的条件下，主要产物是二甲基胍。改变反应条件下形成的产物的分析表明，二甲基叶绿素形成是一种需要解离酶 - 单甲基化的TRNA中间体的两步方法。

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