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首页> 外文期刊>Journal of Virology >Detection of latency-related viral RNAs in trigeminal ganglia of rabbits latently infected with herpes simplex virus type 1.
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Detection of latency-related viral RNAs in trigeminal ganglia of rabbits latently infected with herpes simplex virus type 1.

机译：用疱疹病毒1型兔三叉甘神经节潜伏期相关病毒RNA检测。

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摘要

Using a combination of in situ hybridization and Northern (RNA) blot analysis, we investigated herpes simplex virus type 1 (HSV-1) transcriptional activity in an ocular rabbit model of HSV-1 latency. Radioactively labeled cloned fragments, representing virtually the entire HSV-1 genome, were individually hybridized to RNA in sections of trigeminal ganglia taken from rabbits during the latent phase of infection with HSV-1 (McKrae). Our results suggest that two discrete latency-related RNAs (LR-RNAs) may be present. The LR-RNAs were localized mainly in the nuclei of neurons. The more abundant LR-RNA was detected in approximately 3% of all neurons examined and was designated major LR-RNA. The other LR-RNA, designated minor LR-RNA, was detected in approximately 0.3% of neurons from latently infected rabbits. The genes for the LR-RNAs mapped in the vicinity of the immediate-early gene ICP0 (also designated IE110). The gene for the major LR-RNA partially overlapped the left (3') end of the ICP0 gene. In situ hybridization with single-stranded RNA probes showed that this LR-RNA was of complementary sense to that of ICP0 mRNA. Northern blot analysis gave an approximate size for this LR-RNA of 1.8 to 2.2 kilobases. The minor LR-RNA mapped to or near the right (5') end of the ICP0 gene. The detection of LR-RNAs suggests the possibility that these RNAs or their products may play significant roles in the initiation and/or maintenance of HSV-1 latency.
机译：使用原位杂交和北部（RNA）印迹分析的组合，我们研究了HSV-1等待时间的眼兔模型中的单纯疱疹病毒类型1（HSV-1）转录活性。基于HSV-1（McKrae）的潜在感染潜在的患者，在整个HSV-1基因组几乎是整个HSV-1基因组的放射性标记的克隆片段与来自兔子的三叉肠神经节的RNA单独杂交。我们的结果表明，可以存在两个离散延迟相关的RNA（LR-RNA）。 LR-RNA主要是神经元细胞元的局部。检测到越丰富的LR-RNA在检查的所有神经元的约3％中检测到并被指定为主要的LR-RNA。其他LR-RNA指定的次要LR-RNA，以潜伏的兔的约0.3％的神经元检测到。 LR-RNA的基因映射在立即早期基因ICP0（也指定IE110）附近。主要LR-RNA的基因部分重叠ICP0基因的左（3'）末端。原位与单链RNA探针杂交表明，该LR-RNA对ICP0 mRNA的互补意义。 Northern印迹分析为该LR-RNA提供了1.8至2.2千碱基的近似尺寸。次次LR-RNA映射到ICP0基因的右侧（5'）末端或附近。 LR-RNA的检测表明，这些RNA或其产品可能在HSV-1延迟的启动和/或维持中起显着作用。

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