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首页> 外文期刊>Journal of Virology >Native and recombinant herpes simplex virus type 1 envelope proteins induce human immune T-lymphocyte responses.
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Native and recombinant herpes simplex virus type 1 envelope proteins induce human immune T-lymphocyte responses.

机译：原生和重组疱疹单纯疱疹病毒类型1包络蛋白诱导人类免疫T淋巴细胞反应。

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The abilities of whole herpes simplex virus type 1 (HSV-1) antigen (HSV-ag) and purified HSV-1 native and recombinant envelope proteins to stimulate in vitro T-lymphocyte responses were compared in patients with recurrent herpes labialis. Immunochemically purified preparations of native glycoproteins B, C, and D (ngB, ngC, ngD) from cultured HSV-1 as well as expressed recombinant plasmid preparations of gD (rgD-1t, rgD-45K) elicited lymphocyte proliferation (LT) and production of gamma interferon (IFN-gamma) and interleukin-2 (IL-2) only in seropositive individuals. The IFN-gamma induced by rgD-1t correlated with the time to the next herpetic lesion in 19 volunteers followed to recurrence (r = 0.69, P less than 0.008), although the magnitude and frequency of LT and IFN-gamma responses were lower with either recombinant or native purified antigens than with the whole-virus antigen. Combinations of ngB plus ngD or ngB plus ngC plus ngD stimulated more IFN-gamma, equivalent to whole-virus-antigen responses. Recombinant-derived human IL-2 also specifically increased LT and IFN-gamma responses in antigen-driven cultures. ngD stimulated IL-2 and LT responses similar to those of whole-virus antigen and higher than those of ngC. HSV-ag and ngB induced significantly higher titers of total IFN than could be accounted for by IFN-gamma; this was not seen for the other antigens, which induced only IFN-gamma. HSV-ag-driven Leu 2a-, plastic-nonadherent blood cells, unlike whole peripheral blood mononuclear cells, showed evidence of an increase and then a decline in the frequency of HSV-responsive cells after a lesion recurrence. These studies suggest that HSV-1 envelope proteins are capable of stimulating an immune T-helper-cell response which is associated with the prevention of human herpes simplex lesion recurrence. Although the whole virus probably contains additional important antigens, increasing concentrations or combinations of certain purified glycoproteins or the addition of nonspecific enhancers of T-lymphocyte function can drive in vitro immune responses to the same level as the complete set of viral antigens.
机译：在患有复发性疱疹的患者的患者中，将整个疱疹病毒1（HSV-1）抗原（HSV-1）抗原（HSV-1）抗原（HSV-AG）和纯化的HSV-1天然和重组包膜蛋白质进行了刺激的患者。来自培养的HSV-1的天然糖蛋白B，C和D（NGB，NGC，NGD）的免疫化学纯化制剂以及表达的GD（RGD-1T，RGD-45K）引发淋巴细胞增殖（LT）和生产的重组质粒制剂仅在血清阳性个体中γ干扰素（IFN-Gamma）和白细胞介素-2（IL-2）。 RGD-1T诱导的IFN-GAMMA与19副志愿者中的下一个注重疱疹的时间相关（r = 0.69，p小于0.008），但LT和IFN-γ反应的幅度和频率较低重组或天然纯化的抗原小于全病毒抗原。 NGB Plus NGD或NGB Plus NGC Plus NGD的组合刺激了更多的IFN-γ，相当于全病毒 - 抗原反应。重组衍生的人IL-2也特别增加了抗原驱动培养物中的LT和IFN-Gamma反应。 NGD刺激IL-2和与全部病毒抗原的响应类似，高于NGC。 HSV-AG和NGB诱导总IFN的显着更高的滴度，而不是IFN-Gamma的滴度;这对其他抗原没有看到，仅诱导IFN-Gamma。 HSV-AG驱动的Leu 2A-，塑料非血细胞，与整个外周血单核细胞不同，显示出增加的证据，然后在病变复发后的HSV响应细胞频率下降。这些研究表明，HSV-1包膜蛋白能够刺激免疫T辅助细胞响应，该细胞响应与预防人类单纯疱疹病变复发有关。虽然整个病毒可能含有额外的重要抗原，但是浓度或某些纯化的糖蛋白的组合或添加的T淋巴细胞功能的非特异性增强剂可以在与完整的病毒抗原的相同水平上促进体外免疫应答。

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