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首页> 外文期刊>Journal of Virology >Characterization of the trans-activation-responsive element of the parvovirus H-1 P38 promoter.
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Characterization of the trans-activation-responsive element of the parvovirus H-1 P38 promoter.

机译：细分病毒H-1 P38启动子逆激活响应元件的表征。

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摘要

The parvovirus early protein NS1 positively regulates the expression of the P38 promoter for the viral capsid protein gene. We have examined the trans-activation of P38 by NS1 by using fusions of P38 to the reporter gene, chloramphenicol acetyltransferase (cat). Maximal trans-activation requires a small 5' cis element (tar) between -137 and -116. The tar element has activity in both orientations when 5' to the P38 promoter, but no activity has been detected 3' to the promoter. The wild-type P38 has a biphasic response to NS1 depending on the dosage of the NS1-expressing plasmid. Promoters lacking the tar also have a biphasic response that is reduced about 10-fold, and they can be inhibited by larger doses of the NS1 plasmid. Heterologous promoters from other viruses and the Harvey-ras oncogene promoter are inhibited by NS1. Truncated and internally deleted versions of NS1 lose the trans-activation, but some of them retain the inhibitory properties. Thus transactivation can be uncoupled from inhibition. The tar element has shown no activity with the heterologous simian virus 40 early promoter. In contrast, the P38 promoter responds to a heterologous enhancer, but the enhanced promoter loses activity to trans-activation by NS1. In summary, the P38 tar element has some of the properties of an enhancer with a high preference for a 5' position and a stringent requirement for the P38 promoter.
机译：Parvovirus早期蛋白质NS1积极调节P38启动子对病毒衣壳蛋白基因的表达。通过使用P38的融合向报告基因，氯霉素乙酰转移酶（猫）检查了NS1对NS1进行了P38的跨活化。最大跨激活需要-137和-116之间的小5'顺式元素（焦油）。焦油元素在5'到P38启动子时的定向活动，但没有任何活动被检测到启动子。野生型P38对NS1具有双色反应，这取决于表达NS1的质粒的剂量。缺乏焦油的启动子也具有减少约10倍的双相反应，并且可以通过较大剂量的NS1质粒来抑制它们。来自其他病毒的异源启动子和哈维-Ras癌基因启动子被NS1抑制。截断和内部删除的NS1版本失去了跨激活，但其中一些保留了抑制性质。因此，反式激活可以从抑制中解耦。焦油元素显示出与异源Simian病毒40早期启动子没有活性。相反，P38启动子对异源增强剂作出反应，但增强的启动子失去了NS1的反式激活活性。总之，P38焦油元素具有高偏好的增强子的一些性质，对于5英尺的位置和P38启动子的严格要求。

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