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首页> 外文期刊>Vitis >High embryogenic ability and plant regeneration of table grapevine cultivars (Vitis vinifera L.) induced by activated charcoal.
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High embryogenic ability and plant regeneration of table grapevine cultivars (Vitis vinifera L.) induced by activated charcoal.

机译：活性炭诱导的食用葡萄品种（Vitis vinifera L.）的高胚发生能力和植物再生。

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Somatic embryos and plants from immature anthers or ovaries were obtained from grape cultivars Sugraone, Crimson Seedless, Italia and Don Mariano. Explants were cultured on MS medium supplemented with 2,4-D (4, 7 or 10 micro M) and benzyladenine (0.7, 1 or 1.3 micro M) for callus induction. Callus frequency depended on genotype, explant type and the culture medium used. Calluses were transferred to half strength MS without growth regulators for embryo differentiation. The presence of activated charcoal (AC, 0.25%) in this medium was essential to obtain somatic embryos in the case of Crimson Seedless, Italia and Don Mariano and to increase the frequency of embryogenic calluses in Sugraone (from 5.8% without AC to 99.5% with AC). Ovaries and anthers showed different degrees of embryogenic competence. When somatic embryos were placed in a medium with IAA (10 micro M), GA3 (1 micro M) and 0.25% AC, embryo germination was normal (embryos turned green), the hypocotyls and cotyledons started to grow and the apical root axis developed. The percentage of germinated embryos was 100% for Sugraone and 27.5%, 38.1% and 54.5% for Don Mariano, Italia and Crimson Seedless, respectively. When the germinated embryos were transferred to half strength MS medium in test tubes, 100% of Crimson, Italia and Don Mariano and 68.3% of Sugraone embryos developed into normal plants..
机译：来自未成熟花药或卵巢的体细胞胚和植物是从葡萄品种Sugraone，Crimson Seedless，Italia和Don Mariano获得的。将外植体在补充有2,4-D（4、7或10 microM）和苄基腺嘌呤（0.7、1或1.3 micro M）的MS培养基上培养以诱导愈伤组织。愈伤组织的频率取决于基因型，外植体类型和使用的培养基。将愈伤组织转移至无生长调节剂的半强度MS进行胚胎分化。在深红无核，意大利和唐·马里亚诺的情况下，这种培养基中活性炭（AC，0.25％）的存在对于获得体细胞胚和使Sugraone中的胚性愈伤组织发生频率增加（从无AC的5.8％增至99.5％）至关重要。使用AC）。卵巢和花药显示出不同程度的胚胎发生能力。将体细胞胚置于含有IAA（10 micro M），GA3（1 micro M）和0.25％AC的培养基中时，胚萌发正常（胚变成绿色），下胚轴和子叶开始生长，并且根尖轴发育。 Sugraone的发芽胚百分比为100％，Don Mariano，Italia和Crimson Seedless的发芽胚百分比分别为27.5％，38.1％和54.5％。当将发芽的胚胎转移到试管中的半强度MS培养基中时，100％的Crimson，Italia和Don Mariano和68.3％的Sugraone胚胎发育成正常植物。

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