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Pathogenesis-related proteins in grapevines induced by salicylic acid and Botrytis cinerea

机译：水杨酸和灰葡萄孢诱导葡萄中病原相关蛋白

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The grapevine pathogenesis-related proteins (PRs) were studied in order to determine the spatial localization and the temporal production of these inducible proteins. We used leaves of plants obtained from woody cuttings grown in greenhouse. Elicitations were done either with salicylic acid or with strains of Botrytis cinerea. Several PRs extractable at pH 2.8 were found to accumulate in grapevine leaves after salicylic acid treatment or Botrytis infection (SDS-PAGE, coomassie blue). Elicitation withsalicylic acid has induced one new protein at about 32 kDa. Botrytis infection has resulted in the accumulation of four major acid-soluble proteins with apparent molecular weights of 27, 32, 34 and 38 kDa. Immunodetections using antisera raised againstthe tobacco PR-2 family have shown several bands, particularly two bands at 34 and 36 kDa revealed by the anti-2a and present both with salicylic acid and Botrytis.
机译：为了确定这些可诱导蛋白的空间定位和时间产生，研究了与葡萄发病相关的蛋白（PRs）。我们使用了从温室中生长的木屑获得的植物叶子。用水杨酸或灰葡萄孢菌株进行诱导。在水杨酸处理或葡萄孢感染后，发现在pH 2.8时可提取的几种PR积累在葡萄叶片中（SDS-PAGE，考马斯蓝）。水杨酸的激发已经诱导了一种约32kDa的新蛋白质。葡萄孢菌感染已导致表观分子量分别为27、32、34和38 kDa的四种主要的酸溶性蛋白积累。使用针对烟草PR-2家族产生的抗血清进行的免疫检测显示出多个条带，尤其是抗2a显露的两个分别在34和36 kDa的条带，并与水杨酸和葡萄孢菌同时存在。

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