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首页> 外文期刊>In Vitro Cellular & Developmental Biology - Plant >Bioreactor application on adventitious root culture of Astragalus membranaceus
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Bioreactor application on adventitious root culture of Astragalus membranaceus

机译：生物反应器在黄芪不定根培养中的应用

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Astragalus membranaceus is one of the most widely used traditional medicinal herbs in China, but the time required to generate a useful product in the field production is long. The growth of adventitious root cultures was compared between cultures grown in solid, liquid, or a 5-L balloon-type bubble bioreactor. The maximum growth ratio (final dry weight/initial dry weight) was determined for adventitious roots grown in the bioreactor. Studies carried out to optimize biomass production of adventitious roots compared adventitious root growth from various inoculum root lengths, inoculum densities, and aeration volume in the bioreactors. The maximum growth ratio occurred in treatments with a 1.5-cm inoculum root length, with 30 g (fresh weight) of inoculum per bioreactor or with an aeration volume of 0.1 vvm (air volume/culture medium volume per min). The polysaccharide, saponin, and flavonoid content of roots from bioreactor-grown cultures were compared to roots from field-grown plants grown for 1 and 3 yr. Total polysaccharide content of adventitious roots in the bioreactor (30.0 mg g−1 dry weight (DW)) was higher than the roots of 1-yr-old (13.8 mg g−1 DW) and 3-yr-old (21.1 mg g−1 DW) plants in the field. Total saponin (3.4 mg g−1 DW) and flavonoid (6.4 mg g−1 DW) contents were nearly identical to 3-yr-old roots and higher than that of 1-yr-old roots under field cultivation.
机译：黄芪是中国使用最广泛的传统草药之一，但在田间生产中产生有用产品所需的时间很长。比较了不定根培养物在固体，液体或5-L气球型气泡生物反应器中的生长情况。确定在生物反应器中生长的不定根的最大生长率（最终干重/初始干重）。为优化不定根生物量生产而进行的研究比较了不同反应根长度，接种密度和生物反应器中通气量引起的不定根生长。最大生长比发生在接种根长为1.5厘米，每个生物反应器接种量为30克（鲜重）或通气量为0.1 vvm（每分钟空气量/培养基体积）的处理中。将来自生物反应器生长的培养物的根与来自生长了1年和3年的田间生长植物的根的多糖，皂苷和类黄酮含量进行了比较。生物反应器中不定根的总多糖含量（30.0 mg g -1 干重（DW））高于1岁大麦的根部（13.8 mg g -1） sup> DW）和3岁（21.1 mg g -1 DW）植物。总皂甙（3.4 mg g -1 DW）和类黄酮（6.4 mg g -1 DW）的含量与3岁大根基本相同，高于3岁根。 1岁大的根在田间种植。

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