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首页> 外文期刊>Waste Management >Comparative analysis for the production of fatty acid alkyl esterase using whole cell biocatalyst and purified enzyme from Rhizopus oryzae on waste cooking oil (sunflower oil)
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Comparative analysis for the production of fatty acid alkyl esterase using whole cell biocatalyst and purified enzyme from Rhizopus oryzae on waste cooking oil (sunflower oil)

机译：使用全细胞生物催化剂和米根霉在废食用油（葵花籽油）上纯化的酶生产脂肪酸烷基酯酶的比较分析

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摘要

The petroleum fuel is nearing the line of extinction. Recent research and technology have provided promising outcomes to rely on biodiesel as the alternative and conventional source of fuel. The use of renewable source - vegetable oil constitutes the main stream of research. In this preliminary study, Waste Cooking Oil (WCO) was used as the substrate for biodiesel production. Lipase enzyme producing fungi Rhizopus oryzae 262 and commercially available pure lipase enzyme were used for comparative study in the production of Fatty Acid Alkyl Esters (FAAE). The whole cell (RO 262) and pure lipase enzyme (PE) were immobilized using calcium alginate beads. Calcium alginate was prepared by optimizing with different molar ratios of calcium chloride and different per cent sodium alginate. Entrapment immobilization was done for whole cell biocatalyst (WCB). PE was also immobilized by entrapment for the transe-steriflcation reaction. Seven different solvents - methanol, ethanol, n-propanol, n-butanol, iso-propanol. iso-butanol and iso-amyl alcohol were used as the acyl acceptors. The reaction parameters like temperature (30 ℃), molar ratio (1:3 - oihsolvent), reaction time (24 h), and amount of enzyme (10% mass ratio to oil) were also optimized for methanol alone. The same parameters were adopted for the other acyl acceptors too. Among the different acyl acceptors - methanol. whose reaction parameters were optimized showed maximum conversion of triglycerides to FME-94% with PE and 84% with WCB. On the whole, PE showed better catalytic converting ability with all the acyl acceptor compared to WCB. Gas chromatography analysis (GC) was done to determine the fatty acid composition of WCO (sunflower oil) and FAAE production with different acyl acceptors.
机译：石油燃料已接近灭绝线。最近的研究和技术已经取得了令人鼓舞的成果，那就是依靠生物柴油作为替代燃料和常规燃料。使用可再生资源-植物油构成了研究的主流。在这项初步研究中，废食用油（WCO）被用作生产生物柴油的基质。用于产生脂肪酶的真菌米根霉262和可商购的纯脂肪酶被用于脂肪酸烷基酯（FAAE）的生产中的比较研究。使用藻酸钙珠固定整个细胞（RO 262）和纯脂肪酶（PE）。通过用不同摩尔比的氯化钙和不同百分比的海藻酸钠进行优化来制备海藻酸钙。对全细胞生物催化剂（WCB）进行了固定化。 PE还通过包埋而被固定用于转酯化反应。七种不同的溶剂-甲醇，乙醇，正丙醇，正丁醇，异丙醇。异丁醇和异戊醇用作酰基受体。还单独针对甲醇优化了反应参数，如温度（30℃），摩尔比（1：3-石油溶剂），反应时间（24 h）和酶的量（与油的质量比为10％）。其他酰基受体也采用了相同的参数。在不同的酰基受体中-甲醇。对其反应参数进行了优化，结果表明，使用PE时甘油三酸酯最大转化为FME-94％，使用WCB时甘油转化为FME-94％。总体而言，与所有WCB相比，PE在所有酰基受体上均表现出更好的催化转化能力。进行了气相色谱分析（GC），以确定WCO（葵花籽油）的脂肪酸组成和具有不同酰基受体的FAAE的产量。

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