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首页> 外文期刊>International journal of green energy >The Kinetics of Interesterfication on Waste Cooking Oil (Sunflower Oil) for the Production of Fatty Acid Alkyl Esters using a Whole Cell Biocatalyst (Rhizopus oryzae) and Pure Lipase Enzyme
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The Kinetics of Interesterfication on Waste Cooking Oil (Sunflower Oil) for the Production of Fatty Acid Alkyl Esters using a Whole Cell Biocatalyst (Rhizopus oryzae) and Pure Lipase Enzyme

机译：使用全细胞生物催化剂（米根霉）和纯脂肪酶生产废食用油（向日葵油）的酯交换动力学，用于生产脂肪酸烷基酯

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Recent research and technology have provided promising outcomes to rely on biodiesel as the alternative and conventional source of fuel. The use of renewable sources constitutes the main stream of research. Waste Cooking Oil (WCO) was used for biodiesel production in this study. Lipase enzyme producing fungi Rhizopus oryzae 262 and commercially available pure lipase enzyme were used for comparative study in the production of FAAE. The whole cell biocatalyst and pure enzyme were immobilized using calcium alginate beads. It was prepared by optimizing with different molar ratios of calcium chloride and different percent sodium alginate. Entrapment immobilization was done for whole cell biocatalyst. PE was also immobilized by entrapment for the transesterification reaction. Four different solvents methanol, ethanol, n-propanol, n-butanol were used as the acyl acceptors. The reaction parameters like temperature, molar ratio, reaction time, and amount of enzyme to be used were also optimized for methanol alone. The same parameters were adopted for the other acyl acceptors too. Among the different acyl acceptors, methanol whose reaction parameters were optimized showed maximum conversion of triglycerides to FAAE -94% with PE and 84% with WCB. On the whole, PE showed better catalytic converting ability with all the acyl acceptor compared to WCB, further study, it was observed that three consecutive and reversible reactions occurred in the interesterification of triglycerides. So, a kinetic model based on Michaelis-Menten equation with competitive substrate inhibition was used to find the maximum reaction rate V-i for the four solvents using pure enzyme and WCB.
机译：最近的研究和技术已经取得了令人鼓舞的成果，那就是依靠生物柴油作为替代燃料和常规燃料。使用可再生资源构成了研究的主流。在这项研究中，将废烹饪油（WCO）用于生产生物柴油。使用脂肪酶生产真菌米根霉262和市售的纯脂肪酶进行FAAE生产的比较研究。使用藻酸钙珠固定全细胞生物催化剂和纯酶。通过用不同摩尔比的氯化钙和不同百分比的海藻酸钠进行优化来制备。对全细胞生物催化剂进行了包埋固定。 PE也通过包埋而固定用于酯交换反应。四种不同的溶剂甲醇，乙醇，正丙醇，正丁醇用作酰基受体。仅针对甲醇也优化了反应参数，如温度，摩尔比，反应时间和所用酶的量。其他酰基受体也采用了相同的参数。在不同的酰基受体中，反应参数经过优化的甲醇显示，甘油三酸酯最大转化为FAAE -94％（PE）和84％（WCB）。总体而言，与所有WCB相比，PE在所有酰基受体上均表现出更好的催化转化能力，进一步研究发现，在甘油三酸酯的酯交换反应中发生了三个连续且可逆的反应。因此，使用基于Michaelis-Menten方程并具有竞争性底物抑制的动力学模型，使用纯酶和WCB求出四种溶剂的最大反应速率V-i。

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