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首页> 外文期刊>Water Research >Effect of TiO_2 and CeO_2 nanoparticles on the metabolic activity of surficial sediment microbial communities based on oxygen microelectrodes and high-throughput sequencing
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Effect of TiO_2 and CeO_2 nanoparticles on the metabolic activity of surficial sediment microbial communities based on oxygen microelectrodes and high-throughput sequencing

机译：基于氧微电极和高通量测序的TiO_2和CeO_2纳米粒子对表层沉积物微生物群落代谢活性的影响

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摘要

AbstractEnvironmental concerns regarding the potential ecological risks of metallic oxide nanoparticles (MNPs) in aquatic ecosystems are increasing; sediment is considered a sink for these MNPs. Although several studies have studied the potential impact of MNPs on microbial communities in freshwater and estuarine sediments, limited information is available regarding the influence of MNPs on the metabolic activity of surficial sediment microbial communities and related biogeochemical conditions. To address these issues, a microcosm approach was established to study the metabolic response of surficial sediment microbial communities to a single addition of TiO2or CeO2NPs (5 mg/L) using oxygen microelectrodes, enzyme activity measurements, and high-throughput sequencing. Rapid sedimentation of MNPs (regardless of NP type) was observed in freshwater samples, and most (up to 85%) accumulated in surface sediments (<5 mm). Microelectrode profile measurements in pre-incubated sediments treated with MNPs showed that the oxygen concentration decreased at a slower rate with increasing sediment depth compared to that in untreated controls. Biological oxygen consumption in the uppermost sediment layer (0–1500 μm) was significantly inhibited by MNPs, as calculated from steady-state microprofiles, with CeO2NPs resulting in enhanced acute toxicity than TiO2NPs. High-throughput sequencing showed that MNP exposure increased the bacterial diversity and altered the bacterial community structure, regardless of NP type. The abundance of three dominant bacterial genera,Methylotenera,Cytophagceae_uncultured(classified as an aerobic bacterium), andCyanobacteria_norank(a facultative bacterium), was markedly reduced by MNPs, which was primarily responsible for inhibiting microbial-mediated oxygen consumption in surficial sediments. In summary, short-term exposure to MNPs negatively affected the metabolic activity of benthic microbial communities, which could influence the biogeochemical functions along the sediment-water interface.Graphical abstractDisplay OmittedHighlights•We tested the effect of MNPs on microbial metabolic response in sediments.•Oxygen concentration decreased at a slower rate with increasing sediment depth.•Biological oxygen consumption was inhibited by MNPs resulting in enhanced toxicity.•MNP exposure increased bacterial diversity and altered community structure.•MNPs negatively affected the metabolic activity of benthic microbial communities.
机译： 摘要 对水生生态系统中金属氧化物纳米颗粒（MNP）潜在生态风险的环境关注正在增加；沉积物被认为是这些MNP的汇。尽管一些研究已经研究了MNPs对淡水和河口沉积物中微生物群落的潜在影响，但是关于MNPs对表面沉积物微生物群落的代谢活性和相关生物地球化学条件的影响的信息有限。为了解决这些问题，建立了一个微观方法来研究表层沉积物微生物群落对单添加TiO 2 或CeO 2 NPs（5 mg / L）使用氧气微电极，酶活性测量和高通量测序。在淡水样品中观察到MNP的快速沉降（无论NP类型如何），并且大部分（高达85％）沉积在表面沉积物中（<5 mm）。与未经处理的对照组相比，用MNPs处理的预温育沉积物的微电极轮廓测量表明，随着沉积物深度的增加，氧浓度降低的速度较慢。从稳态微观剖面计算，MNPs显着抑制了最上层沉积层（0-1500μm）的生物耗氧量，其中CeO 2 NPs导致比TiO 2 NPs增强的急性毒性。高通量测序表明，与NP类型无关，MNP暴露会增加细菌多样性并改变细菌群落结构。 Methylotenera ， Cytophagceae_uncultured （分类为有氧细菌）和 Cyanobacteria_norank 三个主要细菌属的丰度ceitalic>（兼性细菌）被MNPs明显减少，而MNPs主要是抑制表层沉积物中微生物介导的氧气消耗。总之，短期接触MNP会对底栖微生物群落的代谢活性产生负面影响，这可能会影响沉积物-水界面的生物地球化学功能。 图形摘要 省略显示 突出显示 • 我们测试了MNP对沉积物中微生物代谢反应的影响。 • 随着沉积物深度的增加，氧气浓度下降的速度较慢。 • MNP抑制了生物耗氧量 • MNP暴露可增加细菌多样性并改变群落结构。 •< / ce：label> MNP对底栖微生物群落的代谢活性产生负面影响。

著录项

  • 来源
    《Water Research》 |2018年第1期|287-296|共10页
  • 作者

    Lingzhan Miao; Peifang Wang; Chao Wang; Jun Hou; Yu Yao; Jun Liu; Bowen Lv; Yangyang Yang; Guoxiang You; Yi Xu; Zhilin Liu; Songqi Liu;

  • 作者单位

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    State Key Laboratory of Biocontrol, Key Laboratory of Biodiversity Dynamics and Conservation of Guangdong Higher Education Institutes, College of Ecology and Evolution, Sun Yat-sen University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

    Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bacterial diversity; Metabolic activity; Metallic oxide nanoparticles; Oxygen consumption; Surficial sediments;

    机译：细菌多样性;代谢活性;金属氧化物纳米颗粒;耗氧量;表层沉积物;

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