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Effects of wavelengths of medium-pressure ultraviolet radiation on photolyase and subsequent photoreactivation

机译：中压紫外线辐射波长对光合酶和随后光活化的影响

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摘要

This study aims to investigate the effect of different wavelengths (254, 266, 280 and 365 nm) in polychromatic medium-pressure (MP) UV radiation on the ability of photolyases in repairing dimers and discusses its impact on subsequent photoreactivation. Photolyase was exposed to various doses and irradiances of the UV wavelengths and the dimer repair abilities of the irradiated photolyase were determined via a spectrophotometric assay. At wavelengths below 300 nm, dimer repair rates were not influenced by the UV irradiation between 0.03 and 0.10 mW cm~2. For 365 nm, photolyase exhibited enhanced dimer repair at 0.05 mW cm~(-2) and then reduced dimer repair with increasing irradiance. In addition, photolyase was found to have decreasing dimer repair rates when exposed to increasing UV doses at all tested wavelengths. Lower photoreactivation levels after MP UV disinfection as compared to low-pressure (LP) UV disinfection was not attributable to a single wavelength in the polychromatic radiation, but is possibly due to the simultaneous exposure of photolyase to a broad spectrum of radiation, which led to a reduction in the dimer repair ability of photolyase. This study is the first to report the direct effects of UV radiation on photolyase enzyme. The data in the study provide some evidence for the mechanism for which MP UV disinfection suppresses photoreactivation in Escherichia coli, which has only been speculated on so far. The knowledge from this study will provide a basis upon which to investigate other enzymes involved in the repair of UV damage to DNA.
机译：这项研究旨在调查多色中压（MP）紫外线辐射中不同波长（254、266、280和365 nm）对光解酶修复二聚体能力的影响，并讨论其对随后光活化的影响。将光解酶暴露于各种剂量和紫外线波长的辐照下，并通过分光光度测定法测定辐照的光解酶的二聚体修复能力。在低于300 nm的波长下，二聚体修复率不受0.03和0.10 mW cm〜2之间的紫外线辐射的影响。对于365 nm，光解酶在0.05 mW cm〜（-2）处显示出增强的二聚体修复，然后随着辐照度的增加而降低了二聚体修复。另外，当在所有测试波长下暴露于增加的紫外线剂量时，发现光裂解酶的二聚体修复率降低。与低压（LP）紫外线消毒相比，MP紫外线消毒后的光活化水平较低，并非归因于多色辐射中的单一波长，而是可能是由于光解酶同时暴露于广谱辐射而导致的。降低了光解酶的二聚体修复能力。这项研究是第一个报告紫外线辐射对光解酶的直接影响的研究。该研究中的数据为MP UV消毒抑制大肠杆菌中光活化的机理提供了一些证据，到目前为止，这一机理尚未被推测出来。这项研究的知识将为研究其他与修复紫外线对DNA的损伤有关的酶提供基础。

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