...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Water science & technology >Characterization of bacterial diversity in drinking water by pyrosequencing
【24h】

Characterization of bacterial diversity in drinking water by pyrosequencing

机译：焦磷酸测序法测定饮用水中细菌的多样性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Controlling microbial contamination of drinking water is critical to public health. However, understanding of the microbial ecology of drinking water remains incomplete. Representing the first application of high-throughput sequencing in drinking water microbiology, the objective of this study is to evaluate pyrosequencing as a high-throughput technique for the characterization of bacterial diversity in drinking water in comparison with conventional clone library analysis. Pyrosequencing and clone library analysis were performed in parallel to study the bacterial community composition in drinking water samples following the concentration of microbial biomass in drinking water with ultrafiltration. Validated by clone library analysis, pyrosequencing was confirmed as a highly efficient deep-sequencing technique to characterize the bacterial diversity in drinking water. Sequences of Alphaproteobacteria and Betaproteobacteria dominated the bacterial community in drinking water with Oxalobacteraceae and Methylobacteriaceae as the most abundant bacterial families, which is consistent with the prominent abundance of these populations frequently detected in various freshwater environments where source waters originate. Bacterial populations represented by the most abundant sequences in drinking water were closely related to cultures of metabolically versatile bacterial taxa widely distributed in the environment, suggesting a potential link between environmental distribution, metabolic characteristics, and abundance in drinking water.
机译：控制饮用水的微生物污染对公众健康至关重要。但是，对饮用水的微生物生态学的了解仍然不完整。代表高通量测序在饮用水微生物学中的首次应用，本研究的目的是与传统的克隆文库分析相比，将焦磷酸测序作为一种用于表征饮用水中细菌多样性的高通量技术进行评估。并行进行焦磷酸测序和克隆文库分析，以研究饮用水中细菌生物素的浓度，并通过超滤对饮用水样品中的细菌群落组成进行研究。通过克隆文库分析验证，焦磷酸测序已被证实是一种高效的深度测序技术，可表征饮用水中的细菌多样性。 Alphaproteobacteria和Betaproteobacteria的序列以饮用水中的细菌群落为主，其中草酸杆菌科和Methylobacteriaceae是最丰富的细菌家族，这与在起源水的各种淡水环境中经常发现的这些种群的丰富性相一致。饮用水中最丰富的序列代表的细菌种群与广泛分布在环境中的代谢用途广泛的细菌类群的培养密切相关，这表明环境分布，代谢特征和饮用水中的丰度之间存在潜在的联系。

著录项

  • 来源
    《Water science & technology》 |2013年第2期|358-368|共11页
  • 作者

    Yan Zhang; Qiang He;

  • 作者单位

    Department of Civil and Environmental Engineering, The university of Tennessee, Knoxville, Tennessee 37996, USA;

    Department of Civil and Environmental Engineering, The university of Tennessee, Knoxville, Tennessee 37996, USA,Institute for a Secure and Sustainable Environment, The University of Tennessee, Knoxville, Tennessee 37996,USA;

  • 收录信息 美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    diversity; drinking water; microbial ecology; proteobacteria; pyrosequencing; ultrafiltration;

    机译：多样性饮用水;微生物生态学蛋白质细菌焦磷酸测序超滤;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号