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Current Protocols in Chemical Biology

中文名称：当前化学生物学的协议

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1.Kinase-Catalyzed Biotinylation of Peptides, Proteins, and Lysates

机译：激酶催化的肽，蛋白质和裂解物的生物素化
- 作者：Chamara Senevirathne;Keith D. Green;Mary Kay H. Pflum
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第1期
- kinase;
- ATP-biotin;
- biotin;
- phosphoprotein;
- protein labeling;
2.Bioorthogonal Chemical Reporters for Analyzing Protein Sulfenylation in Cells

机译：生物正交化学报道分子，用于分析细胞中的蛋白质亚磺酰化
- 作者：Thu H. Truong;Kate S. Carroll
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第2期
- thiol modification;
- protein sulfenylation;
- redox signaling;
- click chemistry;
- in-gel fluorescence;
- western blotting;
3.High-Throughput RT-PCR for Small-Molecule Screening Assays

机译：用于小分子筛选测定的高通量RT-PCR
- 作者：Joshua A. Bittker
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第1期
- real-time PCR;
- high-throughput screening;
- phenotypic screening;
- qRT-PCR;
- gene expression;
4.Dealing with the Data Deluge: Handling the Multitude of Chemical Biology Data Sources

机译：处理数据泛滥：处理大量化学生物学数据源
- 作者：Rajarshi Guha;Dac-Trung Nguyen;Noel Southall;Ajit Jadhav
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第3期
- database;
- federation;
- integration;
- Pubchem;
- HTS;
5.Selecting, Acquiring, and Using Small Molecule Libraries for High-Throughput Screening

机译：选择，获取和使用小分子文库进行高通量筛选
- 作者：Sivaraman Dandapani;Gerard Rosse;Noel Southall;Joseph M. Salvino;Craig J. Thomas
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第3期
- small molecule library;
- high-throughput screening;
- chemical biology;
- drug discovery;
6.Gene Assembly from Chip-Synthesized Oligonucleotides

机译：芯片合成的寡核苷酸的基因组装
- 作者：Nikolai Eroshenko;Sriram Kosuri;Adam H. Marblestone;Nicholas Conway;George M. Church
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第1期
- oligonucleotide;
- gene synthesis;
- nucleic acids;
- synthetic biology;
7.High-Throughput Phosphoproteomics from Formalin-Fixed, Paraffin-Embedded Tissues

机译：福尔马林固定，石蜡包埋的组织的高通量磷酸化蛋白质组学
- 作者：Angelo Gámez-Pozo;Iker Sánchez-Navarro;Nuria Ibarz Ferrer;Fernando García Martínez;Keith Ashman;Juan Angel Fresno Vara
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第2期
- FFPE samples;
- phosphoproteomics;
- biomarkers;
- immobilized metal ion affinity chromatography;
- mass spectrometry;
8.One-Pot Multienzyme Synthesis of Lewis x and Sialyl Lewis x Antigens

机译：Lewis X和Sialyl Lewis x抗原的一锅多酶合成
- 作者：Hai Yu;Kam Lau;Yanhong Li;Go Sugiarto;Xi Chen
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第3期
- enzymatic synthesis;
- FKP;
- fucoside;
- fucose;
- fucosylation;
- fucosyltransferase;
- Lewis x;
- one-pot multienzyme;
- sialyl Lewis x;
9.Measurement of Protein Phosphorylation Stoichiometry by SRM-MS

机译：通过SRM-MS测定蛋白质磷酸化化学计量
- 作者：Lily L. Jin;Yaroslav Sydorskyy;Jiefei Tong;Paul Taylor;Michael F. Moran;Jonathan R. St-Germain
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第1期
- mass spectrometry;
- SRM;
- MRM;
- phosphorylation;
- AQUA;
- stoichiometry;
- label-free;
10.Metallomics Using Inductively Coupled Plasma Mass Spectrometry

机译：使用电感耦合等离子体质谱法的冶金学
- 作者：Brian J. Vaccaro;Angeli L. Menon;W. Andrew Lancaster;Michael W. W. Adams
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第3期
- metalloproteome;
- metallome;
- ICP-MS;
- metalloprotein;
- metalloenzyme;
- bioinorganic chemistry;
- metallobiochemistry;
11.In Situ Hybridization Assay-Based Small-Molecule Screening in Zebrafish

机译：基于原位杂交测定的斑马鱼小分子筛选
- 作者：Lili Jing;Ellen M. Durand;Catherine Ezzio;Stephanie M. Pagliuca;Leonard I. Zon
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第2期
- zebrafish;
- in situ hybridization;
- small molecule screen;
- drug discovery;
- in vivo;
12.Labeling Substrate Proteins of Poly(ADP-ribose) Polymerases with Clickable NAD Analog

机译：用可点击的NAD类似物标记聚（ADP-核糖）聚合酶的底物蛋白
- 作者：Hong Jiang;Hening Lin
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第1期
- clickable NAD analog;
- poly(ADP-ribose) polymerase;
- poly(ADP-ribosyl)ation;
- click chemistry;
13.Nanostructure-Initiator Mass Spectrometry (NIMS) for the Analysis of Enzyme Activities

机译：用于酶活性分析的纳米结构引发剂质谱（NIMS）
- 作者：Wolfgang Reindl;Kai Deng;Xiaoliang Cheng;Anup K. Singh;Blake A. Simmons;Paul D. Adams;Trent R. Northen
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第2期
- nanostructure-initiator mass spectrometry;
- NIMS;
- Nimzyme;
- enzyme assay;
- glycoside hydrolase;
- lignocellulosic biofuel;
14.On-Bead Two-Color (OBTC) Cell Screen for Direct Identification of Highly Selective Cell Surface Receptor Ligands

机译：珠上两色（OBTC）细胞屏幕可直接鉴定高度选择性的细胞表面受体配体
- 作者：D. Gomika Udugamasooriya;Thomas Kodadek
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第1期
- combinatorial;
- cell screen;
- receptor;
- peptoid;
15.Functional Assays on High-Content Protein Microarrays

机译：高含量蛋白质微阵列的功能测定
- 作者：Lihao Meng
- 期刊名称：《Current Protocols in Chemical Biology》 | 2012年第3期
16.Profiling Native Kinases by Immuno-Assisted Activity-Based Profiling

机译：通过基于免疫的活动分析对天然激酶进行分析
- 作者：Eric Okerberg;Ann Shih;Heidi Brown;Senait Alemayehu;Arwin Aban;Tyzoon K. Nomanbhoy
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第3期
- activity based protein profiling;
- immuno-assisted activity based profiling;
- native kinases;
- inhibitors;
17.Penicillin-Binding Protein Imaging Probes

机译：青霉素结合蛋白成像探针
- 作者：Ozden Kocaoglu;Erin E. Carlson
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第4期
- penicillin-binding proteins;
- peptidoglycan;
- fluorescence imaging;
- activity-based probes;
- beta-lactams;
18.Saturation Transfer Difference NMR forFragment Screening

机译：用于片段筛选的饱和传递差异NMR
- 作者：DarrenW. Begley;Spencer O. Moen;Phillip G. Pierce;Edward R. Zartler
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第4期
- saturation transfer difference;
- STD;
- NMR;
- fragment screening;
- fragment-based hit generation;
19.Design, Synthesis, and Application of the Trimethoprim-Based Chemical Tag for Live-Cell Imaging

机译：基于甲氧苄啶的活细胞成像化学标签的设计，合成和应用
- 作者：Chaoran Jing;Virginia W. Cornish
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第2期
- chemical tag;
- fluorescence microscopy;
- live-cell imaging;
- protein label;
20.Using a Kinase-Inducible Bimolecular Switch to Control Enzyme Activity in Living Cells

机译：使用激酶诱导的双分子开关来控制活细胞中的酶活性
- 作者：Sohum Mehta;Jin Zhang
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第3期
- live-cell imaging;
- biochemical perturbation;
- biosensor;
- fluorescence;
21.Bioorthogonal Profiling of ProteinMethylation (BPPM) Using an AzidoAnalog of S-Adenosyl-L-Methionine

机译：使用S-腺苷-L-蛋氨酸的叠氮基类似物对蛋白质甲基化（BPPM）进行生物正交分析
- 作者：Gil Blum;Kabirul Islam;Minkui Luo
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第1期
- G9a;
- protein engineering;
- synthetic cofactor;
- click chemistry;
22.Visualization of O-GlcNAc GlycosylationStoichiometry and Dynamics UsingResolvable Poly(ethylene glycol)Mass Tags

机译：可视化的O-GlcNAc糖基化化学计量学和动力学使用可分辨的聚（乙二醇）质量标签
- 作者：Peter M. Clark;Jessica E. Rexach;Linda C. Hsieh-Wilson
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第4期
23.Determining Lectin Specificity from Glycan Array Data Using Motif Segregation and GlycoSearch Software

机译：使用Motif分离和GlycoSearch软件从聚糖阵列数据确定凝集素特异性
- 作者：Doron Kletter;Zheng Cao;Marshall Bern;Brian Haab
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第2期
- glycobiology;
- glycan array;
- glycan motif;
- lectin;
24.Advances in Lectin MicroarrayTechnology: Optimized Protocols forPiezoelectric Print Conditions

机译：凝集素微阵列技术的进展：压电打印条件的优化协议
- 作者：Kanoelani T. Pilobello;Praveen Agrawal;Richard Rouse;Lara K. Mahal
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第1期
- carbohydrate analysis;
- glycomics;
- lectin microarray;
- Nano-Plotter;
- piezoelectric;
25.In Vivo Imaging and BiochemicalCharacterization of Protease FunctionUsing Fluorescent Activity-Based Probes

机译：使用基于荧光活性的探针对蛋白酶功能进行体内成像和生化表征
- 作者：Laura E. Edgington;Matthew Bogyo
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第1期
- proteases;
- activity-based probes;
- imaging;
- caspases;
26.Phage Selection of Peptide ‘ ‘Microantibodies’ ’

机译：肽“微抗体”的噬菌体选择
- 作者：Daisuke Fujiwara;Ikuo Fujii
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第3期
- phage display;
- peptide;
- protein-protein interaction;
- microantibody;
27.Spatiotemporal Control of Gene Expression in Mammalian Cells and in Mice Using the LightOn System

机译：使用LightOn系统时空控制哺乳动物细胞和小鼠中的基因表达
- 作者：Xianjun Chen;Xue Wang;Zengmin Du;Zhengcai Ma;Yi Yang
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第2期
- LightOn system;
- light induced;
- gene expression;
- mammalian cell;
- mice;
28.Chemiluminescent Nitrogen Detection(CLND) to Measure Kinetic AqueousSolubility

机译：化学发光氮检测（CLND）以测量动力学水溶液的溶解度
- 作者：Aimee Kestranek;Andrew Chervenak;Justin Longenberger;Steven Placko
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第4期
- solubility;
- drug discovery;
- chemiluminescent nitrogen detection;
- physicochemical properties;
- shake flask;
- nephelometry;
29.Multiplexing High-Content Flow (HCF) and Quantitative High-Throughput Screening (qHTS) to Identify Compounds Capable of Decreasing Cell Viability, Activating Caspase 3/7, Expressing Annexin V, and Changing Mitochondrial Membrane Integrity

机译：复用高含量流（HCF）和定量高通量筛选（qHTS），以鉴定能够降低细胞生存力，激活Caspase 3/7，表达膜联蛋白V和改变线粒体膜完整性的化合物
- 作者：Lesley A. Mathews;Jonathan M. Keller;Crystal McKnight;Sam Michael;Paul Shinn;Dongbo Liu;Louis M. Staudt;Craig J. Thomas;Marc Ferrer
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第3期
30.Chemoenzymatic Site-Specific ReversibleImmobilization and Labeling of Proteinsfrom Crude Cellular Extract WithoutPrior Purification Using Oxime andHydrazine Ligation

机译：化学酶特定位点的可逆固定化和粗制细胞提取物中蛋白质的标记，无需事先使用肟和肼连接纯化
- 作者：Mohammad M. Mahmoodi;Mohammad Rashidian;Jonathan K.Dozier;Mark D. Distefano
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第2期
- PFTase;
- farnesyl diphosphate;
- site-specific protein modification;
- protein immobilization;
- oxime ligation;
- hydrazine ligation;
31.Profiling Protein Methylation withCofactor Analog Containing TerminalAlkyne Functionality

机译：用含末端炔烃功能的辅因子类似物分析蛋白质甲基化
- 作者：Gil Blum;Ian R. Bothwell;Kabirul Islam;Minkui Luo
- 期刊名称：《Current Protocols in Chemical Biology》 | 2013年第1期
- Se-adenosyl-L-selenomethionine;
- ProSeAM;
- azido-azo biotin probe;
- click chemistry;
32.High-Throughput Screening for NovelAnti-Infectives Using a C. elegansPathogenesis Model

机译：使用秀丽隐杆线虫发病机理模型的新型抗感染药的高通量筛选
- 作者：Annie L. Conery;Jonah Larkins-Ford;Frederick M. Ausube;Natalia V. Kirienko
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第1期
- Caenorhabditis elegans;
- Pseudomonas aeruginosa;
- high-throughputscreening;
- pathogenesis model;
- antibiotic;
- antimicrobial;
- drug discovery;
- liquid killing;
33.Glycomic Analysis of Glycans Released from Glycoproteins Using Chemical Immobilization and Mass Spectrometry

机译：使用化学固定和质谱法对糖蛋白释放的聚糖进行糖类分析
- 作者：Shuang Yang;Hui Zhang
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第3期
- solid-phase;
- protein immobilization;
- glycan derivatization;
- chemoenzymatic processes;
- MALDI;
- GIG;
- mass spectrometry;
- glycomics;
34.Manipulating Natural Product Biosynthetic Pathways via DNA Assembler

机译：通过DNA组装器操纵天然产物的生物合成途径
- 作者：Zengyi Shao;Huimin Zhao
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第2期
- natural product;
- gene cluster;
- regulation of natural product biosynthesis;
- pathway engineering;
- synthetic biology;
- cryptic pathway;
- DNA assembler;
35.Quantification of Protein Kinase Enzymatic Activity in Unfractionated Cell Lysates Using CSox-Based Sensors

机译：使用基于CSox的传感器对未分级分离的细胞裂解物中的蛋白激酶酶活性进行定量
- 作者：Jon R. Beck;Laura B. Peterson;Barbara Imperiali;Cliff I. Stains
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第3期
- cell signaling;
- kinase activity assay;
- kinase activity profiling;
- phosphorylation;
- fluorescence-based biosensor;
36.Synthesis of Hydrogen-Bond Surrogate alpha-Helices as Inhibitors of Protein-Protein Interactions

机译：氢键替代α-螺旋的合成作为蛋白质-蛋白质相互作用的抑制剂
- 作者：Stephen E. Miller;Paul F. Thomson;Paramjit S. Arora
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第2期
- alpha-helix mimetics;
- hydrogen-bond surrogate;
- protein-protein interactions;
37.Rapid Assays for Lectin Toxicity and Binding Changes that Reflect Altered Glycosylation in Mammalian Cells

机译：凝集素毒性和结合变化的快速测定，反映哺乳动物细胞中糖基化的改变
- 作者：Pamela Stanley;Subha Sundaram
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第2期
- glycosylation mutants;
- mammalian cells;
- engineer glycans;
- glycan binding;
- lectins;
- antibodies;
- CHO cells;
38.Luciferase Reporter Assay in Drosophilaand Mammalian Tissue Culture Cells

机译：果蝇和哺乳动物组织培养细胞中的萤光素酶记者测定
- 作者：Chi Yun;Ramanuj Das Gupta
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第1期
- luciferase;
- reporter gene assay;
- high-throughput screening;
- RNAi;
39.A Biocompatible 'Split Luciferin' Reaction and Its Application for Non-Invasive Bioluminescent Imaging of Protease Activity in Living Animals

机译：生物相容性“分裂萤光素”反应及其在动物体内蛋白酶活性非侵袭性生物发光成像中的应用
- 作者：Aurelien Godinat;Ghyslain Budin;Alma R. Morales;Hyo Min Park;Laura E. Sanman;Matthew Bogyo;Allen Yu;Andreas Stahl;Elena A. Dubikovskaya
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第3期
- protease activity;
- bioocompatible reaction;
- non-invasive;
- bioluminescent imaging;
- in vivo;
40.Probing Small-Molecule Microarrays with Tagged Proteins in Cell Lysates

机译：用细胞裂解物中的标记蛋白探测小分子微阵列
- 作者：Marius S. Pop;Dina Wassaf;Angela N. Koehler
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第4期
- small-molecule microarray;
- binding assay;
- cellular lysate;
- fluorescence;
- protein complex;
41.Determination of the Cytostatic and Cytocidal Activities of Antimalarial Compounds and Their Combination Interactions

机译：抗疟疾化合物的细胞抑制和杀细胞活性及其组合相互作用的测定
- 作者：Katy S. Sherlach;Paul D. Roepe
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第4期
- Plasmodium falciparum;
- antiplasmodial activity;
- cytostatic;
- cytocidal;
- drug combination analysis;
42.Label-Free Cell-Based Dynamic MassRedistribution Assays

机译：基于无标签细胞的动态质量重分布测定
- 作者：Hannah J. Gitschier;Audrey B. Bergeron;David H. Randle
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第1期
43.Nanolithography Based on Metalized DNA Templates for Graphene Patterning

机译：基于金属化DNA模板的纳米光刻技术用于石墨烯构图
- 作者：Zhong Jin;Wei Sun;Peng Yin;Michael S. Strano
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第2期
- DNA self-assembly;
- DNA metalization;
- templated nanolithography;
- masked etching;
- graphene nanostructure patterning;
44.Real-Time Chemiluminescence Imaging Using Nano-Lantern Probes

机译：使用纳米灯笼探针的实时化学发光成像
- 作者：Yoshiyuki Arai;Takeharu Nagai
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第4期
- Nano-lantern;
- Nano-lantern (Ca~(2+));
- optogenetics;
- channel-rhodopsin2 (ChR2);
- dead time;
- CCD camera;
45.A One-Step Imaging Assay to MonitorCell Cycle State and Apoptosis inMammalian Cells

机译：一步成像检测以监测哺乳动物细胞的细胞周期状态和凋亡
- 作者：Yangzhong Tang
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第1期
- high-content screening;
- imaging assay;
- mitosis;
- apoptosis;
- doseresponse;
- pharmacology;
46.Evaluation of Compounds in Primary Human Islet Cell Culture

机译：人体胰岛原代细胞培养物中化合物的评价
- 作者：Deepika Walpita;Bridget K. Wagner
- 期刊名称：《Current Protocols in Chemical Biology》 | 2014年第3期
- pancreatic beta cell;
- islets of Langerhans;
- primary cell culture system;
- high-throughput screening;
- diabetes;
47.In Vivo Delivery of Nitric Oxide-Sensing,Single-Walled Carbon Nanotubes

机译：一氧化氮传感，单壁碳纳米管的体内递送
- 作者：Nicole M. Iverson;Michael S. Strano;Gerald N. Wogan
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第2期
- in vivo;
- single-walled carbon nanotubes;
- nitric oxide;
- sensor;
- intravenous subcutaneous;
- hydroge;
48.Diffraction-Unlimited Fluorescence Microscopy of Living Biological Samples Using pcSOFI

机译：使用pcSOFI的活生物样品的无限制衍射荧光显微镜
- 作者：Sam Duwe;Benjamien Moeyaert;Peter Dedecker
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第1期
- super-resolution microscopy;
- reversible photoswitchable fluorescent proteins;
- pcSOFI;
- 3D imaging;
- fluorescence imaging;
49.Genetic Code Expansion of Mammalian Cells with Unnatural Amino Acids

机译：带有非天然氨基酸的哺乳动物细胞的遗传密码扩展
- 作者：Kalyn A. Brown;Alexander Deiters
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第3期
- unnatural amino acid;
- genetic code expansion;
- mammalian cell;
- caging;
- optogenetics;
- protein translocation;
- nuclear localization;
50.Corking Nitrogen-Doped Carbon Nanotube Cups with Gold Nanoparticles for Biodegradable Drug Delivery Applications

机译：金纳米颗粒的软木塞氮掺杂碳纳米管杯，用于可生物降解的药物输送应用
- 作者：Seth C. Burkert;Alexander Star
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第4期
51.Chemical Synthesis of Ubiquitin Chains

机译：泛素链的化学合成
- 作者：Hosahalli P. Hemantha;Somasekhar Bondalapati;Sumeet K. Singh;Ashraf Brik
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第4期
- ubiquitin;
- ubiquitin chains;
- SPPS;
- ligation;
- desulfurization;
52.Molecular Evolution Directs Protein Translation Using Unnatural Amino Acids

机译：分子进化指导使用非天然氨基酸的蛋白质翻译
- 作者：Vanessa E. Cox;Eric A. Gaucher
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第4期
- unnatural amino acid;
- directed evolution;
- protein translation;
- ancestral proteins;
- protein engineering;
53.Point by Point: An Introductory Guide toSample Preparation for Single-Molecule,Super-Resolution FluorescenceMicroscopy

机译：点对点：单分子超高分辨率荧光显微镜样品制备入门指南
- 作者：Aaron R. Halpern;Marco D. Howard;Joshua C. Vaughan
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第2期
- super-resolution microscopy;
- STORM;
- PALM microtubulesperoxisomes;
- clathrin;
54.Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) to Monitor Compound Effects on Cardiac Myocyte Signaling Pathways

机译：使用人类诱导的多能干细胞衍生的心肌细胞（hiPSC-CMs）监测化合物对心肌信号通路的影响
- 作者：Liang Guo;Sandy Eldridge;Mike Furniss;Jodie Mussio;Myrtle Davis
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第3期
55.A Bead-Based Proximity Assay for BRD4 Ligand Discovery

机译：基于珠子的BRD4配体发现的邻近分析
- 作者：Justin M. Roberts;James E. Bradner
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第4期
- bromodomain;
- BRD4;
- JQ1;
- AlphaScreen;
- high-throughput screening;
- protein-protein interaction;
56.SNIPER Peptide-Mediated Degradation of Endogenous Proteins

机译：SNIPER肽介导的内源蛋白降解
- 作者：Xuelai Fan;YuTianWang
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第1期
- peptide;
- lysosome;
- protein knockdown;
- chaperone-mediated autophagy;
57.SELMA: Selection with ModifiedAptamers

机译：SELMA：使用修饰的适体进行选择
- 作者：J. Sebastian Temme;Isaac J. Krauss
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第2期
- SELMA;
- SELEX;
- HIV;
- in vitro aptamer selection;
- proteincarbohydrate interaction;
58.Identifying Direct Protein Targets ofPoly-ADP-Ribose Polymerases (PARPs)Using Engineered PARP Variants- Orthogonal Nicotinamide AdenineDinucleotide (NAD+) Analog Pairs

机译：使用工程PARP变体-正交烟酰胺腺嘌呤二核苷酸（NAD +）类似物对鉴定ADP核糖核酸聚合酶（PARP）的直接蛋白质靶标
- 作者：Ian Carter-O’Connell;Michael S. Cohen
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第2期
- poly-ADP-ribose polymerase;
- PARP ADP-ribosylation ADPribose;
- ADPr;
- click chemistry;
- proteins;
- post-translational modification;
59.Digestion, Purification, and Enrichment of Protein Samples for Mass Spectrometry

机译：质谱分析蛋白质样品的消化，纯化和富集
- 作者：Victoria E. Hedrick;Mercedes N. LaLand;Ernesto S. Nakayasu;Lake N. Paul
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第3期
- peptides;
- phosphopeptide enrichment;
- protein extraction;
- fractionation;
- on-bead digestion;
- in-gel digestion;
60.Preparation and Separation of DNA-Wrapped Carbon Nanotubes

机译：DNA包裹碳纳米管的制备与分离
- 作者：Geyou Ao;Ming Zheng
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第1期
- carbon nanotube purification;
- ATP system;
- DNA sequences;
- single-chirality;
- modulating agents;
61.Universal Non-Antibody Detection of tl:Protein Phosphorylation Using pIMAGO

机译：使用pIMAGO的tl：蛋白质磷酸化的通用非抗体检测
- 作者：Anton B. Iliuk;W. Andy Tao
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第1期
- phosphoprotein detection;
- phosphorylation;
- ELISA;
- western blot;
- kinase assay;
- high-throughput screening;
62.Biosensing with Virus Electrode Hybrids

机译：病毒电极杂交体的生物传感
- 作者：Kritika Mohan;Reginald M. Penner;Gregory A. Weiss
- 期刊名称：《Current Protocols in Chemical Biology》 | 2015年第2期
- phage display;
- phage wrapping;
- click chemistry;
- prostatespecific membrane;
- antigen electrochemical impedance;
- spectroscopy;
63.Isotope Targeted Glycoproteomics (IsoTaG) to Characterize Intact, Metabolically Labeled Glycopeptides from Complex Proteomes

机译：同位素靶向糖皮质激素（IsoTaG）表征复杂蛋白质组中完整的，经代谢标记的糖肽
- 作者：Christina M. Woo;Carolyn R. Bertozzi
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第1期
- glycoproteomics;
- bioorthogonal chemistry;
- metabolic labeling;
- chemical proteomics;
- mass spectrometry;
64.Reverse Phase Protein Arrays for Compound Profiling

机译：用于化合物谱分析的反相蛋白质阵列
- 作者：Nathan Moerke;Mohammad Fallahi-Sichani
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第3期
- antibodies;
- BRAF;
- cell signaling;
- fluorescence;
- melanoma;
- reverse phase protein arrays;
65.RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells

机译：在生活细菌和哺乳动物细胞中用二聚花椰菜进行RNA成像
- 作者：Grigory S. Filonov;Samie R. Jaffrey
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第1期
- RNA imaging;
- RNA aptamer;
- aptamer expression;
- fluorescence microscopy;
66.Fabrication of 3-D Reconstituted Organoid Arrays by DNA-Programmed Assembly of Cells (DPAC)

机译：通过DNA程序化细胞组装（DPAC）制作3-D重组类器官阵列
- 作者：Michael E. Todhunter;Robert J. Weber;Justin Farlow;Noel Y. Jee;Alec E. Cerchiari;Zev J. Gartner
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第3期
- tissue array;
- DNA;
- synthetic biology;
- cell-cell interactions;
- tissue engineering;
- patterning;
- organotypic;
- organoid;
- 3-D culture;
67.Biochemical, Biophysical and Cellular Techniques to Study the Guanine Nucleotide Exchange Factor, GIV/Girdin

机译：生化，生物物理和细胞技术研究鸟嘌呤核苷酸交换因子GIV / Girdin
- 作者：Pradipta Ghosh;Nicolas Aznar;Lee Swanson;I-Chung Lo;Inmaculada Lopez-Sanchez;Jason Ear;Cristina Rohena;Nicholas Kalogriopoulos;Linda Joosen;Ying Dunkel;Nina Sun
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第4期
- GIV/Girdin;
- trimeric G proteins;
- immunoblotting;
- immunoprecipitation;
- in cellulo GST-pull down;
- immunofluorescence;
- FRET;
68.Nanoparticle-Templated Molecular Recognition Platforms for Detection of Biological Analytes

机译：用于生物分析物检测的纳米粒子模板分子识别平台
- 作者：Abraham G. Beyene;Gozde S. Demirer;Markita P. Landry
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第3期
69.Cyclic Immunofluorescence (CycIF), A Highly Multiplexed Method for Single-cell Imaging

机译：循环免疫荧光（CycIF），一种用于单细胞成像的高度复用方法
- 作者：Jia-Ren Lin;Mohammad Fallahi-Sichani;Jia-Yun Chen;Peter K. Sorger
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第4期
- CycIF;
- immunofluorescence;
- high-content imaging;
- multiplexing;
- systems biology;
70.Hollow-Fiber Methodology for Pharmacokinetic/Pharmacodynamic Studies of Antimalarial Compounds

机译：中空纤维方法用于抗疟药的药代动力学/药效学研究
- 作者：Emily Caton;Elizabeth Nenortas;Rahul P. Bakshi;Theresa A. Shapiro
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第1期
- Plasmodium falciparum;
- pharmacokinetics;
- pharmacodynamics;
- dynamic PK/PD;
- hollow-fiber cartridge system;
- drug development;
- malaria;
71.Pseudo-Ligandless Click Chemistry for Oligonucleotide Conjugation

机译：用于寡核苷酸缀合的伪无配位点击化学
- 作者：Stephanie Mack;Munira F. Fouz;Sourav K. Dey;Subha R. Das
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第2期
- alkyne;
- azide;
- bioconjugation;
- DNA;
- labeling;
- nucleic acids;
- RNA;
72.Tracking the Activity of mTORC1 in Living Cells Using Genetically Encoded FRET-based Biosensor TORCAR

机译：使用基于基因编码的基于FRET的生物传感器TORCAR跟踪活细胞中mTORC1的活性
- 作者：Xin Zhou;Simin Li;Jin Zhang
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第4期
- biosensor;
- fluorescence;
- live-cell imaging;
- mTOR kinase;
73.Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS)

机译：光诱导核定位信号（LINuS）在活细胞中的核蛋白导入的光遗传学控制。
- 作者：Pierre Wehler;Dominik Niopek;Roland Eils;Barbara Di Ventura
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第2期
- LOV2;
- nuclear import;
- optogenetics;
- protein engineering;
- synthetic biology;
74.Liquid Exfoliation of Layered Transition Metal Dichalcogenides for Biological Applications

机译：层状过渡金属二硫属元素化物的液体剥离用于生物应用
- 作者：Emily P. Nguyen;Torben Daeneke;Serge Zhuiykov;Kourosh Kalantar-zadeh
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第2期
- exfoliation;
- functionalization;
- ion intercalation;
- liquid phase exfoliation;
- transition metal dichalcogenides;
75.Combinatorial Library Screening Coupled to Mass Spectrometry to Identify Valuable Cyclic Peptides

机译：组合文库筛选与质谱联用，以鉴定有价值的环肽
- 作者：Silvia A. Camperi;Silvana L. Giudicessi;María C. Martínez-Ceron;Juan M. Gurevich-Messina;Soledad L. Saavedra;Gerardo Acosta;Osvaldo Cascone;Rosa Erra-Balsells;Fernando Albericio
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第2期
- affinity;
- ChemMatrix;
- combinatorial;
- depsipeptides;
- MALDI MS;
76.Enriching s4U-RNA Using Methane Thiosulfonate (MTS) Chemistry

机译：使用甲烷硫代磺酸盐（MTS）化学富集s4U-RNA
- 作者：Erin E. Duffy;Matthew D. Simon
- 期刊名称：《Current Protocols in Chemical Biology》 | 2016年第4期
- metabolic labeling;
- 4-thiouridine;
- methane thiosulfonate;
- RNA metabolism;
77.Elucidating Compound Mechanism of Action and Predicting Cytotoxicity Using Machine Learning Approaches, Taking Prediction Confidence into Account

机译：利用机器学习方法阐明化合物作用机制和预测细胞毒性，考虑预测信心
- 作者：Georgios Drakakis;Isidro Cortes-Ciriano;Ben Alexander-Dann
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第3期
- ChEMBL;
- cytotoxicity;
- in silico bioactivity prediction;
- mechanism of action;
- polypharmacology;
- toxicology modeling;
78.Enzymatic Synthesis of Backbone-Modified Oligonucleotides Using T4 DNA Ligase

机译：使用T4 DNA连接酶酶促合成骨架改性寡核苷酸
- 作者：Donaat Kestemont;Piet Herdewijn;Marleen Renders
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第2期
- backbone-modified oligonucleotides;
- crowding agents;
- enzymatic synthesis;
- mixed-chemistry;
- T4 DNA ligase;
79.Endotoxin-Free Preparation of GrapheneOxide and Graphene-Based Materials forBiological Applications

机译：无石油制剂的石墨毒和基于石墨烯的材料的无毒性应用
- 作者：Dorsa Parviz;Michael Stran
- 期刊名称：《Current Protocols in Chemical Biology》 | 2018年第4期
- colloidal stability;
- endotoxin;
- exfoliation;
- graphene oxide;
- graphene;
- liquid-phase exfoliation;
- oxidation;
80.EndoVIPER‐seq for Improved Detection of A‐to‐I Editing Sites in

机译：Endoviper-SEQ改进了检测A-TO-I编辑网站的检测
- 作者：Cellular RNA;Steve D. Knutson;Jennifer M. Heemstra
- 期刊名称：《Current Protocols in Chemical Biology》 | 2020年第2期
81.Biochemical Stimulation of Immune Cells and Measurement of Mechanical Responses Using Atomic Force Microscopy

机译：使用原子力显微镜的免疫细胞生化刺激和测量机械响应
- 作者：Kenneth H. Hu;Marc A. Bruce;Jianwei Liu
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第2期
- atomic force microscope;
- cantilever;
- mechanical cell forces;
- mechanobiology;
- T cell;
82.In Vitro Activity Assays to Quantitatively Assess the Endogenous Reversible Oxidation State of Protein Tyrosine Phosphatases in Cells

机译：体外活动测定以定量评估细胞中蛋白酪氨酸磷酸酶的内源性可逆氧化状态
- 作者：Avinash D. Londhe;Syed H. M. Rizvi;Benoit Boivin
- 期刊名称：《Current Protocols in Chemical Biology》 | 2020年第3期
83.Detection of Small‐Molecule Aggregation with High‐Throughput Microplate Biophysical Methods

机译：用高通量微孔板生物物理方法检测小分子聚集
- 作者：Samantha J. Allen;Corey M. Dower;Annie X. Liu
- 期刊名称：《Current Protocols in Chemical Biology》 | 2020年第1期
84.Activity-Based Kinome Profiling Using Chemical Proteomics and ATP Acyl Phosphates

机译：基于活性的Kinome分析，使用化学蛋白质组学和ATP酰基磷酸盐
- 作者：Caroline E. Franks;Ku-Lung Hsu
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第3期
- activity-based protein profiling;
- chemical proteomics;
- chemo-proteomics;
- kinase;
- KiNativ;
- kinome;
- mass spectrometry;
85.Synthesis and Application of an Activity-Based Peptide-Peptoid Hybrid Probe for the Immunoproteasome

机译：用于免疫促妇体的活性基肽肽杂交探针的合成与应用
- 作者：Breanna L. Zerfas;Darci J. Trader
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第4期
- activity-based;
- cells;
- fluorescence;
- immunoproteasome;
- probe;
86.Engineering Bacterial Shape Using SoftMatter Microchambers

机译：使用软塑造微型摄影器的工程细菌形状
- 作者：Lars David Renner
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第1期
- bacteria;
- mechanics microbiology;
- microfabrication;
- spheroplasts;
87.Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale

机译：通过光亲和力标记（SIM-PAL）映射的小分子互蛋白映射，以鉴定蛋白质组尺寸的小分子的结合位点
- 作者：Hope A. Flaxman;David K. Miyamoto;Christina M. Woo
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第4期
88.Preparation of Ligand-Targeted DrugConjugates for Cancer Therapy andTheir Evaluation In Vitro

机译：癌症治疗的配体靶向药物缀合物的制备及其在体外评价
- 作者：Mena Asha Krishnan;Sagnik Sengupta;Venkatesh Chelvam
- 期刊名称：《Current Protocols in Chemical Biology》 | 2018年第4期
- cancer therapy;
- drug conjugates;
- in vitro;
- peptide spacer;
- protein biomarker;
- targeting ligand;
89.Quantification of Cellular Proteostasis inLive Cells by Fluorogenic Assay Using theAgHalo Sensor

机译：使用TheaGhalo传感器荧光测定量化细胞蛋白质抑制细胞的定量
- 作者：Matthew Fares;Xin Zhang
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第1期
- fluorescence intensity;
- fluorogenic sensor;
- protein aggregationproteostasis;
90.A Sensitive High-Throughput ScreeningMethod for Identifying Small MoleculeStimulators of the Core Particle of theProteasome

机译：一种敏感的高通量筛选方法，用于识别ProteAsome的核心粒子的小分子刺激剂
- 作者：Rachel A. Coleman;Darci J. Trader
- 期刊名称：《Current Protocols in Chemical Biology》 | 2018年第4期
- peptide;
- proteasome;
- screening;
- stimulation;
91.Ratiometric Single‐Molecule FRET Measurements to Probe Conformational Subpopulations of Intrinsically Disordered Proteins

机译：比率单分子尺寸测量，以探测本机蛋白质的构象亚群
- 作者：Irem Nasir;Emily P. Bentley;Ashok A. Den
- 期刊名称：《Current Protocols in Chemical Biology》 | 2020年第1期
92.Purification and Application of Genetically Encoded Potassium Ion Indicators for Quantification of Potassium Ion Concentrations within Biological Samples

机译：遗传编码钾离子指标在生物样品中定量钾离子浓度的纯化和应用
- 作者：H. Bischof;S. Burgstaller;N. Vujic
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第3期
- cell viability;
- FRET;
- GEPIIs;
- K~+;
- potassium ions;
- recombinant protein;
- serum K~+;
93.In Vivo Identification of Protein KinaseSubstrates by Kinase-Oriented SubstrateScreening (KIOSS)

机译：通过激酶取向底曲线（Kioss）体内鉴定蛋白质动力学质量
- 作者：Tomoki Nishioka;Mutsuki Amano;Yasuhiro Funahashi;Daisuke Tsuboi;Yukie Yamahashi;Kozo Kaibuchi
- 期刊名称：《Current Protocols in Chemical Biology》 | 2019年第1期
- nteractome;
- kinase;
- mass spectrometry;
- phosphorylation;
- phosphoproteomics;
94.High-Throughput Screening of HECT E3 Ubiquitin Ligases Using UbFluor

机译：使用Ubfluor的Hect E3泛素连接酶的高通量筛选
- 作者：Peter K. Foote;David T. Krist;Alexander V. Statsyuk
- 期刊名称：《Current Protocols in Chemical Biology》 | 2017年第3期
- ubiquitin;
- HECT E3 ligase;
- high-throughput screen;
- fluorescence polarization;
- thioester;
95.Overview of Methods and Strategies for Conducting Virtual Small Molecule Screening

机译：进行虚拟小分子筛选的方法和策略概述
- 作者：Xavier Fradera;Kerim Babaoglu
- 期刊名称：《Current Protocols in Chemical Biology》 | 2017年第3期
- virtual screening;
- molecular docking;
- pharmacophore searches;
- ligand-based screening;
96.A Guide to Quantitative Biomarker Assay Development using Whispering Gallery Mode Biosensors

机译：使用耳语画廊模式生物传感器定量生物标志物测定的指南
- 作者：Heather M. Robison;Ryan C. Bailey
- 期刊名称：《Current Protocols in Chemical Biology》 | 2017年第3期
- whispering gallery mode sensors;
- immunoassay;
- biomarker;
- protein detection;
- multiplex;
- quantitative analysis;
97.Characterizing the Nano-Bio Interface Using Microscopic Techniques: Imaging the Cell System is Just as Important as Imaging the Nanoparticle System

机译：使用微观技术表征纳米生物界面：成像电池系统与纳米粒子系统成像一样重要
- 作者：Christie M. Sayes;Henry Lujan
- 期刊名称：《Current Protocols in Chemical Biology》 | 2017年第3期
- nano-bio interface;
- electron microscopy;
- fluorescence microscopy;
- atomic force microcopy;
98.Optimized PEI-based Transfection Method for Transient Transfection and Lentiviral Production

机译：瞬态转染和慢病毒生产的优化PEI转染方法
- 作者：Shaozhe Yang;Xiaoling Zhou;Rongxiang Li;Xiuhong Fu;Pingnan Sun
- 期刊名称：《Current Protocols in Chemical Biology》 | 2017年第3期
- lentivirial production;
- polyethylenimine;
- transfection method;
- transient transfection;
99.Early-Stage Formulation Considerations

机译：早期的制定考虑因素
- 作者：Robert W. Lee;Mark Mitchnick
- 期刊名称：《Current Protocols in Chemical Biology》 | 2017年第4期
- drug discovery;
- new chemical entity;
- new molecular entity;
- preformulation;
- test article vehicle;
100.Evolving Aptamers with Unnatural BasePairs

机译：使用不自然的底座演变的适体
- 作者：Michiko Kimoto;Ken-ichiro Matsunaga;Ichiro Hirao
- 期刊名称：《Current Protocols in Chemical Biology》 | 2017年第4期
- DNA aptamer;
- ExSELEX;
- genetic alphabet expansion;
- SELEX;
- unnatural base pair;

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