• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Physiologia plantarum >A novel fluorescence imaging approach to monitor salt stress-induced modulation of ouabain-sensitive ATPase activity in sunflower seedling roots
【24h】

A novel fluorescence imaging approach to monitor salt stress-induced modulation of ouabain-sensitive ATPase activity in sunflower seedling roots

机译：一种新型的荧光成像方法来监测盐胁迫诱导的向日葵幼苗根中哇巴因敏感性ATPase活性的调节

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Seedlings exposed to salt stress are expected to show modulation of intracellular accumulation of sodium ions through a variety of mechanisms. Using a new methodology, this work demonstrates ouabain (OU)-sensitive ATPase activity in the roots of sunflower seedlings subjected to salt stress (120 mM NaCl). 9-Anthroylouabain (a derivative of ouabain known to inhibit Na+,K+-ATPase activity in animal systems, EC 3.6.3.9) has been used as a probe to analyze OU-sensitive ATPase activity in sunflower (Helianthus annuus) seedling roots by spectrofluorometric estimation and localization of its spatial distribution using confocal laser scanning microscopy. Salt stress for 48 h leads to a significant induction of OU-sensitive ATPase activity in the meristematic region of the seedling roots. Calcium ions (10 mM) significantly inhibit enzyme activity and a parallel accumulation of sodium ions in the cytosol of the columella cells, epidermis and in the cells of the meristematic region of the roots is evident. As a rapid response to NaCl stress, the activity of OU-sensitive ATPase gets localized in the nuclear membrane of root protoplasts and it gets inhibited after treatment with calcium ions. Nuclear membrane localization of the OU-sensitive ATPase activity highlights a possible mechanism to efflux sodium ions from the nucleus. Thus, a correlation between OU-sensitive ATPase activity, its modulation by calcium ions and accumulation of sodium ions in various regions of the seedling roots, has been demonstrated using a novel approach in a plant system.
机译：预计暴露于盐胁迫的幼苗会通过多种机制显示出钠离子在细胞内积累的调节。使用一种新方法，这项工作证明了经受盐胁迫（120 mM NaCl）的向日葵幼苗根中的哇巴因（OU）敏感ATPase活性。 9-Anthroylouabain（一种哇巴因的衍生物，已知能抑制动物系统中的Na +，K + -ATPase活性，EC 3.6.3.9）已被用作探针，通过分光光度法分析向日葵（Helianthus annuus）幼苗根中的OU敏感ATPase活性。使用共聚焦激光扫描显微镜对其空间分布进行定位。持续48小时的盐胁迫导致幼苗根分生组织区域中OU敏感ATPase活性的显着诱导。钙离子（10 mM）会显着抑制酶活性，并且钠离子在小肠细胞，表皮和根的分生组织区域的细胞质中平行积累是明显的。作为对NaCl胁迫的快速响应，OU敏感ATPase的活性位于根系原生质体的核膜中，并在用钙离子处理后被抑制。 OU敏感ATPase活性的核膜定位突出了可能的钠离子从核中流出的机制。因此，已在植物系统中使用新颖的方法证明了OU敏感性ATPase活性，其被钙离子调节和钠离子在幼苗根部各个区域的积累之间的相关性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号