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首页> 外文期刊>Physiological and Molecular Plant Pathology >DIFFERENTIAL INDUCTION AND ACCUMULATION OF BETA-1,3-GLUCANASE AND CHITINASE ISOFORMS IN SOYBEAN HYPOCOTYLS AND LEAVES AFTER COMPATIBLE AND INCOMPATIBLE INFECTION WITH PHYTOPHTHORA MEGASPERMA F SP GLYCINEA
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DIFFERENTIAL INDUCTION AND ACCUMULATION OF BETA-1,3-GLUCANASE AND CHITINASE ISOFORMS IN SOYBEAN HYPOCOTYLS AND LEAVES AFTER COMPATIBLE AND INCOMPATIBLE INFECTION WITH PHYTOPHTHORA MEGASPERMA F SP GLYCINEA

机译：大豆疫霉和大豆疫霉感染后大豆叶型和叶片中β-1，3-葡聚糖酶和几丁质酶同工型的差异诱导和积累

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Phytophthora megasperma f.sp. glycinea infection greatly induced the synthesis and accumulation of beta-1,3-glucanases and chitinases in hypocotyls and leaves of soybean seedlings. Native polyacrylamide gel electrophoresis and isoelectric focusing were used to identify and quantify the isoforms of the two hydrolases in soybean tissues. Some beta-1,3-glucanase isoforms were constitutively expressed in soybeans, but other isoforms only accumulated in soybean or only in a particular organ of soybean after inoculation with P. m. f.sp. glycinea infection or after treatment with mercuric chloride. The pI 8 . 4 isoform, Gb 2 of beta-1,3-glucanase constitutively present in soybean hypocotyls and leaves completely disappeared after mercuric chloride treatment. P. m. f.sp. glycinea infection and mercuric chloride treatment leads to the accumulation of distinctly different isoforms of chitinase in soybean tissues. The acidic chitinase isoform Ca 4 was induced and accumulated in hypocotyls, bur not in leaves, after pathogen infection and mercuric chloride treatment. In general, beta-1,3-glucanase and chitinase isoforms accumulated in the diseased soybean tissues during symptom development in both compatible and incompatible interactions. The results also suggest that pathogen infection and chemical stress may function in a different organ-specific manner to induce isoforms of beta-1,3-glucanase and chitinase in soybeans. (C) 1996 Academic Press Limited.
机译：巨大疫霉甘氨酸感染极大地诱导了大豆幼苗下胚轴和叶片中β-1，3-葡聚糖酶和几丁质酶的合成和积累。天然聚丙烯酰胺凝胶电泳和等电聚焦用于鉴定和定量大豆组织中两种水解酶的同工型。在大豆中，一些β-1,3-葡聚糖酶同工型在大豆中组成性表达，但其他同工型仅在大豆中或仅在大豆的特定器官中积累。 f.sp.甘氨酸感染或氯化汞治疗后。 pI 8。 4种亚型，组成型存在于大豆下胚轴中的β-1,3-葡聚糖酶的Gb 2，在氯化汞处理后完全消失。下午f.sp.甘氨酸感染和氯化汞处理导致大豆组织中几丁质酶的异构体明显不同。在病原体感染和氯化汞处理后，酸性几丁质酶同工型Ca 4被诱导并积累在下胚轴中，而不存在于叶片中。通常，在症状发展期间，在相容和不相容相互作用中，β-1,3-葡聚糖酶和几丁质酶同工型在患病大豆组织中积累。结果还表明，病原体感染和化学胁迫可能以不同的器官特异性方式起作用，从而诱导大豆中β-1,3-葡聚糖酶和几丁质酶的亚型。 （C）1996 Academic Press Limited。

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